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PDBsum entry 2sak
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Plasminogen activator PDB id
2sak

 

 

 

 

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Contents
Protein chain
121 a.a. *
Ligands
TRS
Waters ×85
* Residue conservation analysis
PDB id:
2sak
Name: Plasminogen activator
Title: Staphylokinase (sakstar variant)
Structure: Staphylokinase. Chain: a. Mutation: yes
Source: Staphylococcus aureus. Organism_taxid: 1280
Resolution:
1.80Å     R-factor:   0.181     R-free:   0.237
Authors: A.Rabijns,H.L.De Bondt,M.De Maeyer,I.Lasters,C.De Ranter
Key ref: A.Rabijns et al. (1997). Three-dimensional structure of staphylokinase, a plasminogen activator with therapeutic potential. Nat Struct Biol, 4, 357-360. PubMed id: 9145104
Date:
20-Feb-97     Release date:   25-Feb-98    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P68802  (SAK_STAAU) -  Staphylokinase from Staphylococcus aureus
Seq:
Struc: 		163 a.a.
121 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
Nat Struct Biol 4:357-360 (1997)
PubMed id: 9145104  
 
 
Three-dimensional structure of staphylokinase, a plasminogen activator with therapeutic potential.
A.Rabijns, H.L.De Bondt, C.De Ranter.
 
  ABSTRACT  
 
The three-dimensional structure of staphylokinase has been determined at 1.8 A. The puntative site of interaction with plasminogen was identified and epitopes were mapped.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19214390 A.Apte-Deshpnade, G.Mandal, S.Soorapaneni, B.Prasad, J.Kumar, and S.Padmanabhan (2009).
High-level expression of non-glycosylated and active staphylokinase from Pichia pastoris.
  Biotechnol Lett, 31, 811-817.  
17605815 A.M.Burroughs, S.Balaji, L.M.Iyer, and L.Aravind (2007).
Small but versatile: the extraordinary functional and structural diversity of the beta-grasp fold.
  Biol Direct, 2, 18.  
17919340 H.Chen, W.Mo, H.Su, Y.Zhang, and H.Song (2007).
Characterization of a novel bifunctional mutant of staphylokinase with platelet-targeted thrombolysis and antiplatelet aggregation activities.
  BMC Mol Biol, 8, 88.  
17485856 J.F.Chiou, M.D.Woon, S.N.Cheng, C.H.Hsu, S.C.Cherng, F.K.Hsieh, S.M.Lin, and C.Y.Shiau (2007).
Staphylokinase-annexin XI chimera exhibited efficient in vitro thrombolytic activities.
  Biosci Biotechnol Biochem, 71, 1122-1129.  
17635705 J.L.Degen, T.H.Bugge, and J.D.Goguen (2007).
Fibrin and fibrinolysis in infection and host defense.
  J Thromb Haemost, 5, 24-31.  
16230338 R.Friedrich, P.Panizzi, S.Kawabata, W.Bode, P.E.Bock, and P.Fuentes-Prior (2006).
Structural basis for reduced staphylocoagulase-mediated bovine prothrombin activation.
  J Biol Chem, 281, 1188-1195.
PDB code: 2a1d
16894474 Y.G.Zhang, L.Yue, Y.X.Wang, X.M.Tao, and H.Y.Song (2006).
Design of a novel plasminogen activator based on the structure of hirudin.
  Acta Biochim Biophys Sin (Shanghai), 38, 531-536.  
11856331 Y.Chen, G.Song, F.Jiang, L.Feng, X.Zhang, Y.Ding, M.Bartlam, A.Yang, X.Ma, S.Ye, Y.Liu, H.Tang, H.Song, and Z.Rao (2002).
Crystal structure of a staphylokinase: variant a model for reduced antigenicity.
  Eur J Biochem, 269, 705-711.
PDB codes: 1c77 1c78 1c79
11742690 K.Lähteenmäki, P.Kuusela, and T.K.Korhonen (2001).
Bacterial plasminogen activators and receptors.
  FEMS Microbiol Rev, 25, 531-552.  
11085285 K.Okada, S.Ueshima, M.Tanaka, H.Fukao, and O.Matsuo (2000).
Analysis of plasminogen activation by the plasmin-staphylokinase complex in plasma of alpha2-antiplasmin-deficient mice.
  Blood Coagul Fibrinolysis, 11, 645-655.  
10075668 R.G.Miele, M.Prorok, V.A.Costa, and F.J.Castellino (1999).
Glycosylation of asparagine-28 of recombinant staphylokinase with high-mannose-type oligosaccharides results in a protein with highly attenuated plasminogen activator activity.
  J Biol Chem, 274, 7769-7776.  
  9925575 S.J.Szarka, E.G.Sihota, H.R.Habibi, and S.Wong (1999).
Staphylokinase as a plasminogen activator component in recombinant fusion proteins.
  Appl Environ Microbiol, 65, 506-513.  
9712854 B.Schlott, K.H.Gührs, M.Hartmann, A.Röcker, and D.Collen (1998).
NH2-terminal structural motifs in staphylokinase required for plasminogen activation.
  J Biol Chem, 273, 22346-22350.  
9500599 D.Collen (1998).
Staphylokinase: a potent, uniquely fibrin-selective thrombolytic agent.
  Nat Med, 4, 279-284.  
9692953 O.Ohlenschläger, R.Ramachandran, K.H.Gührs, B.Schlott, and L.R.Brown (1998).
Nuclear magnetic resonance solution structure of the plasminogen-activator protein staphylokinase.
  Biochemistry, 37, 10635-10642.
PDB code: 1ssn
9733510 X.Wang, X.Lin, J.A.Loy, J.Tang, and X.C.Zhang (1998).
Crystal structure of the catalytic domain of human plasmin complexed with streptokinase.
  Science, 281, 1662-1665.
PDB code: 1bml
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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