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PDBsum entry 2php
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Structural genomics, unknown function
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PDB id
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2php
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Structural genomics, unknown function
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Title:
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Crystal structure of thE C-terminal domain of protein mj0236 (y236_metja)
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Structure:
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Uncharacterized protein mj0236. Chain: a, b, d, e. Fragment: residues 240-420. Engineered: yes
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Source:
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Methanocaldococcus jannaschii dsm 2661. Organism_taxid: 243232. Strain: dsm 2661, jal-1, jcm 10045, nbrc 100440. Atcc: 43067. Gene: mj0236. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Resolution:
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2.03Å
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R-factor:
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0.216
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R-free:
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0.238
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Authors:
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S.Eswaramoorthy,S.K.Burley,S.Swaminathan,New York Sgx Research Center For Structural Genomics (Nysgxrc)
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Key ref:
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S.Eswaramoorthy
et al.
Crystal structure of the c-Terminal domain of protein mj0236 (y236_metja).
To be published,
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Date:
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11-Apr-07
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Release date:
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24-Apr-07
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PROCHECK
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Headers
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References
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Q57688
(THIDN_METJA) -
Bifunctional thiamine biosynthesis protein ThiDN from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
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Seq:
Struc:
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417 a.a.
181 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 1:
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E.C.2.5.1.3
- thiamine phosphate synthase.
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Reaction:
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1.
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2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H+ = thiamine phosphate + CO2 + diphosphate
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2.
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2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl- 5-(diphosphooxymethyl)pyrimidine + 2 H+ = thiamine phosphate + CO2 + diphosphate
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3.
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4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine + H+ = thiamine phosphate + diphosphate
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2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate
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+
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4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine
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+
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2
×
H(+)
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=
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thiamine phosphate
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+
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CO2
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+
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diphosphate
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2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
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+
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4-amino-2-methyl- 5-(diphosphooxymethyl)pyrimidine
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+
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2
×
H(+)
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=
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thiamine phosphate
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+
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CO2
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+
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diphosphate
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4-methyl-5-(2-phosphooxyethyl)-thiazole
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+
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4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine
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+
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H(+)
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=
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thiamine phosphate
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+
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diphosphate
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Enzyme class 2:
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E.C.2.7.1.49
- hydroxymethylpyrimidine kinase.
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Reaction:
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4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5- (phosphooxymethyl)pyrimidine + ADP + H+
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4-amino-5-hydroxymethyl-2-methylpyrimidine
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+
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ATP
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=
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2
×
4-amino-2-methyl-5- (phosphooxymethyl)pyrimidine
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+
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ADP
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+
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H(+)
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Enzyme class 3:
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E.C.2.7.4.7
- phosphooxymethylpyrimidine kinase.
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Reaction:
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4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-amino-2-methyl- 5-(diphosphooxymethyl)pyrimidine + ADP
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4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine
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+
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ATP
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=
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2
×
4-amino-2-methyl- 5-(diphosphooxymethyl)pyrimidine
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+
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ADP
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
