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PDBsum entry 2phf
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protein ligands metals Protein-protein interface(s) links
Sugar binding protein PDB id
2phf

 

 

 

 

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Contents
Protein chains
241 a.a. *
Ligands
MAN-MAN
BMA-MAN-MAN-MAN
Metals
_CA ×2
_MN ×2
Waters ×231
* Residue conservation analysis
PDB id:
2phf
Name: Sugar binding protein
Title: Pterocarpus angolensis lectin complexed with man-6
Structure: Lectin. Chain: a, b
Source: Pterocarpus angolensis. Organism_taxid: 182271
Resolution:
2.10Å     R-factor:   0.180     R-free:   0.216
Authors: A.Garcia-Pino,L.Buts,L.Wyns,A.Imberty,R.Loris
Key ref: A.Garcia-Pino et al. (2007). How a plant lectin recognizes high mannose oligosaccharides. Plant Physiol, 144, 1733-1741. PubMed id: 17556509
Date:
11-Apr-07     Release date:   10-Jul-07    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q8GSD2  (Q8GSD2_PTEAG) -  Lectin (Fragment) from Pterocarpus angolensis
Seq:
Struc: 		260 a.a.
241 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 

 
Plant Physiol 144:1733-1741 (2007)
PubMed id: 17556509  
 
 
How a plant lectin recognizes high mannose oligosaccharides.
A.Garcia-Pino, L.Buts, L.Wyns, A.Imberty, R.Loris.
 
  ABSTRACT  
 
The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20574564 E.Säwén, T.Massad, C.Landersjö, P.Damberg, and G.Widmalm (2010).
Population distribution of flexible molecules from maximum entropy analysis using different priors as background information: application to the Φ, Ψ-conformational space of the α-(1-->2)-linked mannose disaccharide present in N- and O-linked glycoproteins.
  Org Biomol Chem, 8, 3684-3695.  
20711856 M.Kandeel, and Y.Kitade (2010).
Substrate specificity and nucleotides binding properties of NM23H2/nucleoside diphosphate kinase homolog from Plasmodium falciparum.
  J Bioenerg Biomembr, 42, 361-369.  
19756298 R.D.Cummings (2009).
The repertoire of glycan determinants in the human glycome.
  Mol Biosyst, 5, 1087-1104.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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