PDBsum entry 2osb

Transferase

PDB id: 2osb

Contents

Protein chains

216 a.a. *

Ligands

AP5 ×2

Metals

_ZN ×2

_MG ×2

Waters ×277

* Residue conservation analysis

PDB id:

2osb

Name:

Transferase

Title:

Crystal structure of a thermostable mutant of bacillus subtilis adenylate kinase (q16l/q199r/)

Structure:

Adenylate kinase. Chain: a, b. Synonym: atp-amp transphosphorylase, ak, superoxide-inducible protein 16, soi16. Engineered: yes. Mutation: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Strain: 168. Gene: adk. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.245 R-free: 0.264

Authors:

J.Myers,R.Counago,C.J.Wilson,G.Wu,Y.Shamoo

Key ref:

R.Counago et al. Crystal structure of a thermostable mutant of bacillus subtilis adenylate kinase (q16l/q199r/). To be published, .

Date:

05-Feb-07 Release date: 15-Jan-08

Protein chains

P16304  (KAD_BACSU) -  Adenylate kinase from Bacillus subtilis (strain 168)

Seq: 217 a.a.

Struc: 216 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.4.3 - adenylate kinase.
• Reaction: AMP + ATP = 2 ADP

AMP + ATP (Bound ligand (Het Group name = AP5) matches with 54.39% similarity) = 2 × ADP

Molecule diagrams generated from .mol files obtained from the KEGG ftp site