PDBsum entry 2os0

Hydrolase

PDB id: 2os0

Contents

Protein chain

179 a.a. *

Ligands

SO4

Metals

_NI

Waters ×273

* Residue conservation analysis

PDB id:

2os0

Name:

Hydrolase

Title:

Structures of actinonin bound peptide deformylases from e. Faecalis and s. Pyogenes

Structure:

Peptide deformylase. Chain: a. Synonym: pdf, polypeptide deformylase. Engineered: yes. Mutation: yes

Source:

Enterococcus faecalis. Organism_taxid: 1351. Strain: atcc 700802. Gene: def. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.30Å R-factor: 0.195 R-free: 0.207

Authors:

E.E.Kim,K.-H.Kim,J.H.Moon,K.Choi,H.K.Lee,H.S.Park

Key ref:

E.E.Kim et al. Structures of actinonin bound peptide deformylases from e. Faecalis and s. Pyogenes. To be published, .

Date:

05-Feb-07 Release date: 04-Mar-08

Protein chain

Q82ZJ0  (DEF_ENTFA) -  Peptide deformylase from Enterococcus faecalis (strain ATCC 700802 / V583)

Seq: 187 a.a.

Struc: 179 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.1.88 - peptide deformylase.
• Reaction: N-terminal N-formyl-L-methionyl-[peptide] + H2O = N-terminal L-methionyl- [peptide] + formate
• Cofactor: Fe(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site