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PDBsum entry 2opy
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Apoptosis activator
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PDB id
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2opy
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Apoptosis activator
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Title:
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Smac mimic bound to bir3-xiap
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Structure:
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Baculoviral iap repeat-containing protein 4. Chain: a. Synonym: inhibitor of apoptosis protein 3, x-linked inhibitor of apoptosis protein, x-linked iap, iap-like protein, hilp. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: birc4, api3, iap3, xiap. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
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Resolution:
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2.80Å
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R-factor:
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0.213
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R-free:
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0.235
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Authors:
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A.D.Wist,G.Lu,S.J.Riedl,Y.Shi,G.L.Mclendon
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Key ref:
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A.D.Wist
et al.
(2007).
Structure-activity based study of the Smac-binding pocket within the BIR3 domain of XIAP.
Bioorg Med Chem Lett,
15,
2935-2943.
PubMed id:
DOI:
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Date:
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30-Jan-07
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Release date:
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20-Feb-07
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PROCHECK
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Headers
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References
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P98170
(XIAP_HUMAN) -
E3 ubiquitin-protein ligase XIAP from Homo sapiens
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Seq:
Struc:
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497 a.a.
106 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.2.3.2.27
- RING-type E3 ubiquitin transferase.
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Reaction:
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S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6- ubiquitinyl-[acceptor protein]-L-lysine
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DOI no:
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Bioorg Med Chem Lett
15:2935-2943
(2007)
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PubMed id:
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Structure-activity based study of the Smac-binding pocket within the BIR3 domain of XIAP.
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A.D.Wist,
L.Gu,
S.J.Riedl,
Y.Shi,
G.L.McLendon.
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ABSTRACT
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A small series of peptide mimics was designed and synthesized to contain a
heterocyclic ring in place of the potentially labile N-terminal peptide bond of
the tetrapeptide containing the Smac-XIAP-binding motif. Two Smac mimics were
shown to bind to the BIR3 domain of XIAP with moderate affinity and one
displayed increased activity in cells relative to the Smac peptides. The
structures of BIR3-XIAP in complex with a Smac peptide and a peptide mimic were
solved and analyzed to elucidate the structure-activity relationship surrounding
the Smac-binding domain within BIR3-XIAP.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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C.D.Moore,
H.Wu,
B.Bolaños,
S.Bergqvist,
A.Brooun,
T.Pauly,
and
D.Nowlin
(2009).
Structural and biophysical characterization of XIAP BIR3 G306E mutant: insights in protein dynamics and application for fragment-based drug design.
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Chem Biol Drug Des,
74,
212-223.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
