PDBsum entry 2nu2

Hydrolase

PDB id: 2nu2

Contents

Protein chains

185 a.a. *

51 a.a. *

Waters ×173

* Residue conservation analysis

PDB id:

2nu2

Name:

Hydrolase

Title:

Accommodation of positively-charged residues in a hydrophobic specificity pocket: crystal structures of sgpb in complex with omtky3 variants lys18i and arg18i

Structure:

Streptogrisin b, protease b. Chain: e. Fragment: residues 115-299. Synonym: sgpb, pronase enzyme b. Ovomucoid. Chain: i. Fragment: third domain, residues 135-185. Engineered: yes. Mutation: yes

Source:

Streptomyces griseus. Organism_taxid: 1911. Strain: k1. Meleagris gallopavo. Turkey. Organism_taxid: 9103. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

1.65Å R-factor: 0.167

Authors:

K.S.Bateman,S.Anderson,W.Lu,M.A.Qasim,M.Laskowski Jr.,M.N.G.James

Key ref:

K.S.Bateman et al. Accommodation of positively-Charged residues in a hydrophobic specificity pocket: crystal structures of sgpb in complex with omtky3 variants lys18i and arg18i. To be published, .

Date:

08-Nov-06 Release date: 21-Nov-06

Protein chain

P00777  (PRTB_STRGR) -  Streptogrisin-B from Streptomyces griseus

Seq: 299 a.a.

Struc: 185 a.a.

Protein chain

P68390  (IOVO_MELGA) -  Ovomucoid from Meleagris gallopavo

Seq: 185 a.a.

Struc: 51 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain E: E.C.3.4.21.81 - streptogrisin B.
• Reaction: Hydrolysis of proteins with specificity similar to trypsin.