PDBsum entry 2mw6

Toxin

PDB id: 2mw6

Contents

Protein chain

27 a.a.

Ligands

3UQ

PDB id:

2mw6

Name:

Toxin

Title:

Structure of the bee venom toxin melittin with [(c5h5)ru]+ fragment attached to the tryptophan residue

Structure:

Melittin. Chain: a. Fragment: residues 44-69. Synonym: allergen api m 3, allergen api m iii

Source:

Apis mellifera. European honey bee,western honey bee,bee,honeybee. Organism_taxid: 7460

NMR struc:

10 models

Authors:

D.S.Perekalin,A.A.Pavlov,V.V.Novikov

Key ref:

D.S.Perekalin et al. (2015). Selective ruthenium labeling of the tryptophan residue in the bee venom Peptide melittin. Chemistry, 21, 4923-4925. PubMed id: 25688543 DOI: 10.1002/chem.201406510

Date:

28-Oct-14 Release date: 01-Jul-15

Protein chain

P01501  (MEL_APIME) -  Melittin from Apis mellifera

Seq: 70 a.a.

Struc: 27 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1002/chem.201406510

Chemistry 21:4923-4925 (2015)

PubMed id: 25688543

Selective ruthenium labeling of the tryptophan residue in the bee venom Peptide melittin.

D.S.Perekalin, V.V.Novikov, A.A.Pavlov, I.A.Ivanov, N.Y.Anisimova, A.N.Kopylov, D.S.Volkov, I.F.Seregina, M.A.Bolshov, A.R.Kudinov.

ABSTRACT

Melittin is a membrane-active peptide from bee venom with promising antimicrobial and anticancer activity. Herein we report on a simple and selective method for labeling of the tryptophan residue in melittin by the organometallic fragment [(C5 H5 )Ru](+) in aqueous solution and in air. Ruthenium coordination does not disturb the secondary structure of the peptide (as verified by 2D NMR spectroscopy), but changes the pattern of its intermolecular interactions resulting in an 11-fold decrease of hemolytic activity. The high stability of the organometallic conjugate allowed the establishment of the biodistribution of the labeled melittin in mice by inductively coupled plasma MS analysis of ruthenium.