PDBsum entry 2mw3

Unknown function

PDB id: 2mw3

Contents

Protein chain

21 a.a.

PDB id:

2mw3

Name:

Unknown function

Title:

Solution nmr structure of the lasso peptide streptomonomicin

Structure:

Lasso peptide. Chain: a

Source:

Streptomonospora alba. Organism_taxid: 183763

NMR struc:

15 models

Authors:

J.I.Tietz,L.Zhu,D.A.Mitchell,M.Metelev,J.O.Melby,P.M.Blair,I.Livnat, K.Severinov

Key ref:

M.Metelev et al. (2015). Structure, bioactivity, and resistance mechanism of streptomonomicin, an unusual lasso Peptide from an understudied halophilic actinomycete. Chem Biol, 22, 241-250. PubMed id: 25601074 DOI: 10.1016/j.chembiol.2014.11.017

Date:

24-Oct-14 Release date: 28-Jan-15

Protein chain

A0A0C2JEQ8  (A0A0C2JEQ8_9ACTN) -  Streptomonomicin peptide protein from Streptomonospora alba

Seq: 41 a.a.

Struc: 21 a.a.

DOI no: 10.1016/j.chembiol.2014.11.017

Chem Biol 22:241-250 (2015)

PubMed id: 25601074

Structure, bioactivity, and resistance mechanism of streptomonomicin, an unusual lasso Peptide from an understudied halophilic actinomycete.

M.Metelev, J.I.Tietz, J.O.Melby, P.M.Blair, L.Zhu, I.Livnat, K.Severinov, D.A.Mitchell.

ABSTRACT

Natural products are the most historically significant source of compounds for drug development. However, unacceptably high rates of compound rediscovery associated with large-scale screening of common microbial producers have resulted in the abandonment of many natural product drug discovery efforts, despite the increasing prevalence of clinically problematic antibiotic resistance. Screening of underexplored taxa represents one strategy to avoid rediscovery. Herein we report the discovery, isolation, and structural elucidation of streptomonomicin (STM), an antibiotic lasso peptide from Streptomonospora alba, and report the genome for its producing organism. STM-resistant clones of Bacillus anthracis harbor mutations to walR, the gene encoding a response regulator for the only known widely distributed and essential two-component signal transduction system in Firmicutes. To the best of our knowledge, Streptomonospora had been hitherto biosynthetically and genetically uncharacterized, with STM being the first reported compound from the genus. Our results demonstrate that understudied microbes remain fruitful reservoirs for the rapid discovery of novel, bioactive natural products.