PDBsum entry 2mpe

Unknown function

PDB id: 2mpe

Contents

Protein chain

130 a.a.

PDB id:

2mpe

Name:

Unknown function

Title:

Solution nmr structure for b. Pseudomallei bpsl1050

Structure:

Bpsl1050. Chain: a. Engineered: yes

Source:

Burkholderia pseudomallei. Organism_taxid: 272560. Strain: k96243. Gene: bpsl1050. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

15 models

Authors:

D.Gaudesi,G.Musco,G.Quilici

Key ref:

D.Gaudesi et al. (2015). Structure-based design of a B cell antigen from B. pseudomallei. Acs Chem Biol, 10, 803-812. PubMed id: 25495888 DOI: 10.1021/cb500831y

Date:

15-May-14 Release date: 11-Mar-15

Protein chain

Q63W39  (Q63W39_BURPS) -  DUF5594 domain-containing protein from Burkholderia pseudomallei (strain K96243)

Seq: 126 a.a.

Struc: 130 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1021/cb500831y

Acs Chem Biol 10:803-812 (2015)

PubMed id: 25495888

Structure-based design of a B cell antigen from B. pseudomallei.

D.Gaudesi, C.Peri, G.Quilici, A.Gori, M.Ferrer-Navarro, O.Conchillo-Solé, R.Thomas, A.Nithichanon, G.Lertmemongkolchai, R.Titball, X.Daura, G.Colombo, G.Musco.

ABSTRACT

Burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in South-East Asia, causing septicemia and organ failure with high mortality rates. Current treatments and diagnostic approaches are largely ineffective. The development of new diagnostic tools and vaccines toward effective therapeutic opportunities against B. pseudomallei is therefore an urgent priority. In the framework of a multidisciplinary project tackling melioidosis through reverse and structural vaccinology, BPSL1050 was identified as a candidate for immunodiagnostic and vaccine development based on its reactivity against the sera of melioidosis patients. We determined its NMR solution structure and dynamics, and by novel computational methods we predicted immunogenic epitopes that once synthesized were able to elicit the production of antibodies inducing the agglutination of the bacterium and recognizing both BPSL1050 and B. pseudomallei crude extracts. Overall, these results hold promise for novel chemical biology approaches in the discovery of new diagnostic and prophylactic tools against melioidosis.