PDBsum entry 2mi8

Signaling protein

PDB id: 2mi8

Contents

Protein chain

76 a.a.

PDB id:

2mi8

Name:

Signaling protein

Title:

Solution structure of lysine-free (k0) ubiquitin

Structure:

Ubiquitin. Chain: a. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ubc. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

T.Huang,J.R.Li,A.R.Byrd

Key ref:

T.Huang et al. (2014). Solution structure of lysine-free (K0) ubiquitin. Protein Sci, 23, 662-667. PubMed id: 24591328 DOI: 10.1002/pro.2450

Date:

09-Dec-13 Release date: 02-Apr-14

Protein chain

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 76 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1002/pro.2450

Protein Sci 23:662-667 (2014)

PubMed id: 24591328

Solution structure of lysine-free (K0) ubiquitin.

T.Huang, J.Li, R.A.Byrd.

ABSTRACT

Lysine-free ubiquitin (K0-Ub) is commonly used to study the ubiquitin-signaling pathway, where it is assumed to have the same structure and function as wild-type ubiquitin (wt-Ub). However, the K0-Ub (15) N heteronuclear single quantum correlation NMR spectrum differs significantly from wt-Ub and the melting temperature is depressed by 19°C, raising the question of the structural integrity and equivalence to wt-Ub. The three-dimensional structure of K0-Ub was determined by solution NMR, using chemical shift and residual dipolar coupling data. K0-Ub adopts the same backbone structure as wt-Ub, and all significant chemical shifts can be related to interactions impacted by the K to R mutations.