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PDBsum entry 2mf2
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PDB id:
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Hydrolase
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Title:
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Structural and biophysical characterization of the mRNA interferase samazf from staphylococcus aureus.
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Structure:
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mRNA interferase mazf. Chain: a, b. Synonym: endoribonuclease mazf, toxin mazf. Engineered: yes
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Source:
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Staphylococcus aureus. Organism_taxid: 1280. Gene: mazf, mw1992. Expressed in: escherichia coli. Expression_system_taxid: 562.
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NMR struc:
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20 models
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Authors:
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V.Zorzini,A.Cheung,R.Loris,N.A.J.Van Nuland
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Key ref:
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V.Zorzini
et al.
(2011).
1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase.
Biomol Nmr Assign,
5,
157-160.
PubMed id:
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Date:
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03-Oct-13
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Release date:
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21-May-14
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PROCHECK
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Headers
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References
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Q2FWI8
(MAZF_STAA8) -
Endoribonuclease MazF from Staphylococcus aureus (strain NCTC 8325 / PS 47)
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Seq:
Struc:
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120 a.a.
132 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Biomol Nmr Assign
5:157-160
(2011)
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PubMed id:
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1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase.
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V.Zorzini,
S.Haesaerts,
A.Cheung,
R.Loris,
N.A.van Nuland.
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ABSTRACT
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MazF proteins are ribonucleases that cleave mRNA with high sequence-specificity
as part of bacterial stress response and that are neutralized by the action of
the corresponding antitoxin MazE. Prolonged activation of the toxin MazF leads
to cell death. Several mazEF modules from gram-negative bacteria have been
characterized in terms of catalytic activity, auto-regulation mechanism and
structure, but less is known about their distant relatives found in
gram-positive organisms. Currently, no solution NMR structure is available for
any wild-type MazF toxin. Here we report the (1)H, (15)N and (13)C backbone and
side-chain chemical shift assignments of this toxin from the pathogen bacterium
Staphylococcus aureus. The BMRB accession number is 17288.
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Links
