PDBsum entry 2map

Transcription/DNA

PDB id: 2map

Contents

Protein chain

96 a.a.

DNA/RNA

PDB id:

2map

Name:

Transcription/DNA

Title:

Solution structure of the complex formed by the region 2 of e. Coli sigmae and its cognate -10 promoter element non template strand tgtcaaa.

Structure:

RNA polymerase sigma factor. Chain: a. Engineered: yes. DNA. Chain: b. Fragment: part of the -10 element non template strand. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 536056. Strain: atcc 33849 / dsm 4235 / ncib 12045 / k12 / dh1. Gene: rpoe, ecdh1_1095, ecdh1me8569_2500. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

NMR struc:

18 models

Authors:

S.Campagne,J.A.Vorholt,F.H.-T.Allain

Key ref:

S.Campagne et al. (2014). Structural basis for -10 promoter element melting by environmentally induced sigma factors. Nat Struct Biol, 21, 269-276. PubMed id: 24531660 DOI: 10.1038/nsmb.2777

Date:

16-Jul-13 Release date: 19-Feb-14

Protein chain

C9R146  (C9R146_ECOD1) -

DNA/RNA chain

T-G-T-C-A-A-A 7 bases

DOI no: 10.1038/nsmb.2777

Nat Struct Biol 21:269-276 (2014)

PubMed id: 24531660

Structural basis for -10 promoter element melting by environmentally induced sigma factors.

S.Campagne, M.E.Marsh, G.Capitani, J.A.Vorholt, F.H.Allain.

ABSTRACT

Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli σ(E), which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.