Nmr solution structure of the d3'-hairpin from the sc.Ai5gamma group ii intron including the ebs1:dibs1 rna:dna hybrid
Structure:
RNA (29-mer). Chain: a. Fragment: d3'ebs1. Engineered: yes. Dna_(5'-d( Cp Ap Gp Tp Gp Tp C)-3')_. Chain: b. Fragment: dibs1. Engineered: yes
Source:
Synthetic: yes. Saccharomyces cerevisiae. Organism_taxid: 4932. Other_details: in vitro transcription from a synthetic double- stranded DNA template using t7 RNA polymerase. Synthetic construct. Organism_taxid: 32630
NMR struc:
18 models
Authors:
M.Skilandat,R.K.O.Sigel
Key ref:
M.Skilandat
and
R.K.Sigel
(2014).
The role of Mg(II) in DNA cleavage site recognition in group II intron ribozymes: solution structure and metal ion binding sites of the RNA-DNA complex.
J Biol Chem,
289,
20650-20663.
PubMed id: 24895129
DOI: 10.1074/jbc.M113.542381
The role of Mg(II) in DNA cleavage site recognition in group II intron ribozymes: solution structure and metal ion binding sites of the RNA-DNA complex.