PDBsum entry 2m0r

Metal binding protein

PDB id: 2m0r

Contents

Protein chain

104 a.a.

PDB id:

2m0r

Name:

Metal binding protein

Title:

Solution structure and dynamics of human s100a14

Structure:

Protein s100-a14. Chain: a, b. Synonym: s100 calcium-binding protein a14, s114. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: s100a14, s100a15. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

30 models

Authors:

I.Bertini,V.Borsi,L.Cerofolini,S.Das Gupta,M.Fragai,C.Luchinat

Key ref:

I.Bertini et al. (2013). Solution structure and dynamics of human S100A14. J Biol Inorg Chem, 18, 183-194. PubMed id: 23197251

Date:

05-Nov-12 Release date: 23-Jan-13

Protein chains

Q9HCY8  (S10AE_HUMAN) -  Protein S100-A14 from Homo sapiens

Seq: 104 a.a.

Struc: 104 a.a.

Enzyme reactions

• Enzyme class: E.C.?

J Biol Inorg Chem 18:183-194 (2013)

PubMed id: 23197251

Solution structure and dynamics of human S100A14.

I.Bertini, V.Borsi, L.Cerofolini, S.Das Gupta, M.Fragai, C.Luchinat.

ABSTRACT

Human S100A14 is a member of the EF-hand calcium-binding protein family that has only recently been described in terms of its functional and pathological properties. The protein is overexpressed in a variety of tumor cells and it has been shown to trigger receptor for advanced glycation end products (RAGE)-dependent signaling in cell cultures. The solution structure of homodimeric S100A14 in the apo state has been solved at physiological temperature. It is shown that the protein does not bind calcium(II) ions and exhibits a "semi-open" conformation that thus represents the physiological structure of the S100A14. The lack of two ligands in the canonical EF-hand calcium(II)-binding site explains the negligible affinity for calcium(II) in solution, and the exposed cysteines and histidine account for the observed precipitation in the presence of zinc(II) or copper(II) ions.