PDBsum entry 2ko0

Transcription/DNA

PDB id: 2ko0

Contents

Protein chain

87 a.a. *

DNA/RNA

Metals

_ZN

* Residue conservation analysis

PDB id:

2ko0

Name:

Transcription/DNA

Title:

Solution structure of the thap zinc finger of thap1 in complex with its DNA target

Structure:

Thap domain-containing protein 1. Chain: a. Fragment: unp residues 1-82. Engineered: yes. Mutation: yes. Rrm1. Chain: b. Engineered: yes. Other_details: specific thap1 binding sequence element found in the

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: thap1. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: de3. Synthetic: yes. Other_details: DNA synthesis.

NMR struc:

15 models

Authors:

S.Campagne,V.Gervais,O.Saurel,A.Milon

Key ref:

S.Campagne et al. (2010). Structural determinants of specific DNA-recognition by the THAP zinc finger. Nucleic Acids Res, 38, 3466-3476. PubMed id: 20144952

Date:

08-Sep-09 Release date: 19-Jan-10

Protein chain

Q9NVV9  (THAP1_HUMAN) -  THAP domain-containing protein 1 from Homo sapiens

Seq: 213 a.a.

Struc: 87 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

DNA/RNA chains

G-C-T-T-G-T-G-T-G-G-G-C-A-G-C-G 16 bases

C-G-C-T-G-C-C-C-A-C-A-C-A-A-G-C 16 bases

Enzyme reactions

• Enzyme class: E.C.?

Nucleic Acids Res 38:3466-3476 (2010)

PubMed id: 20144952

Structural determinants of specific DNA-recognition by the THAP zinc finger.

S.Campagne, O.Saurel, V.Gervais, A.Milon.

ABSTRACT

Human THAP1 is the prototype of a large family of cellular factors sharing an original THAP zinc-finger motif responsible for DNA binding. Human THAP1 regulates endothelial cell proliferation and G1/S cell-cycle progression, through modulation of pRb/E2F cell-cycle target genes including rrm1. Recently, mutations in THAP1 have been found to cause DYT6 primary torsion dystonia, a human neurological disease. We report here the first 3D structure of the complex formed by the DNA-binding domain of THAP1 and its specific DNA target (THABS) found within the rrm1 target gene. The THAP zinc finger uses its double-stranded beta-sheet to fill the DNA major groove and provides a unique combination of contacts from the beta-sheet, the N-terminal tail and surrounding loops toward the five invariant base pairs of the THABS sequence. Our studies reveal unprecedented insights into the specific DNA recognition mechanisms within this large family of proteins controlling cell proliferation, cell cycle and pluripotency.