PDBsum entry 2kld

Membrane protein

PDB id: 2kld

Contents

Protein chain

73 a.a. *

* Residue conservation analysis

PDB id:

2kld

Name:

Membrane protein

Title:

Solution structure of the calcium binding domain of thE C-terminal cytosolic domain of polycystin-2

Structure:

Polycystin-2. Chain: a. Fragment: residues 680-796. Synonym: polycystic kidney disease 2 protein, autosomal dominant polycystic kidney disease type ii protein, polycystwin, r48321. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: pkd2. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

10 models

Authors:

H.R.Kalbitzer

Key ref:

F.H.Schumann et al. (2009). NMR-assignments of a cytosolic domain of the C-terminus of polycystin-2. Biomol Nmr Assign, 3, 141-144. PubMed id: 19636966

Date:

01-Jul-09 Release date: 28-Jul-09

Protein chain

Q13563  (PKD2_HUMAN) -  Polycystin-2 from Homo sapiens

Seq: 968 a.a.

Struc: 73 a.a.

Biomol Nmr Assign 3:141-144 (2009)

PubMed id: 19636966

NMR-assignments of a cytosolic domain of the C-terminus of polycystin-2.

F.H.Schumann, H.Hoffmeister, M.Schmidt, R.Bader, E.Besl, R.Witzgall, H.R.Kalbitzer.

ABSTRACT

Mutations in the PKD2 gene lead to the development of polycystic kidney disease (PKD). The PKD2 gene codes for polycystin-2, a cation channel with unknown function. The cytoplasmic, C-terminal domain interacts with a large number of proteins including mDia1, alpha-actinin, PIGEA-14, troponin, and tropomyosin. The C-terminal fragment polycystin-2 (680-796) consisting of 117 amino acids contains a putative calcium binding EF-hand. It was produced in Escherichia coli and enriched uniformly with (13)C and (15)N. The backbone and side chain resonances were assigned by multidimensional NMR methods, the obtained chemical shifts are typical for a partially folded protein. The chemical shifts obtained are in line with the existence of two paired helix-loop-helix (HLH) motifs.