PDBsum entry 2kfq

De novo protein

PDB id: 2kfq

Contents

Protein chain

32 a.a. *

* Residue conservation analysis

PDB id:

2kfq

Name:

De novo protein

Title:

Nmr structure of fp1

Structure:

Fp1. Chain: a. Synonym: transcription factor sp1. Engineered: yes

Source:

Synthetic: yes. Other_details: peptide synthesis

NMR struc:

10 models

Authors:

M.Araki,A.Tamura

Key ref:

M.Araki and A.Tamura (2009). Solubility-dependent structural formation of a 25-residue, natively unfolded protein, induced by addition of a seven-residue peptide fragment. Febs J, 276, 2336-2347. PubMed id: 19292861

Date:

26-Feb-09 Release date: 31-Mar-09

Protein chain

No UniProt id for this chain

Struc: 32 a.a.

Febs J 276:2336-2347 (2009)

PubMed id: 19292861

Solubility-dependent structural formation of a 25-residue, natively unfolded protein, induced by addition of a seven-residue peptide fragment.

M.Araki, A.Tamura.

ABSTRACT

To elucidate the architectural principle of protein structure, we focused on sequestration from solvent, which is a common characteristic of folding and self-associative precipitation. Because protein solubility can be regarded as a basis for the potential ability to sequester from solvent, we assume that poorly soluble proteins tend not only to precipitate, but also to form solution structures. To examine this, the solubility of a 25-residue, natively unfolded protein, modified from a zinc-finger domain of transcription factor Sp1, was disturbed by adding a seven-residue hydrophobic peptide fragment to the C-terminus. NMR and ultracentrifuge measurements of the resulting sequence showed that a dissolved species forms an alpha-helical structure in a 15-20 molecule oligomer. To elucidate the mechanism by which the structure forms, we prepared two variants in which the added fragments are less hydrophobic; the structural stabilities were then measured at various pH values. A fairly good correlation was observed between stability and hydration potential, whereas a much stronger correlation was observed between stability and solubility, indicating that the stability is more strongly dependent on the ability to precipitate than on dehydration. These results show that, among poorly soluble protein molecules, dissolved species can be transformed from the solvent-exposed unfolded state into a loosely packed structure via intermolecular interactions. Because decreasing the protein solubility does not require the primary sequence to have a sophisticated design, such a protein structure might form readily and frequently, compared with the well-packed structure found in native proteins.