PDBsum entry 2jhc

Viral protein

PDB id: 2jhc

Contents

Protein chain

613 a.a. *

Ligands

GUN ×2

* Residue conservation analysis

PDB id:

2jhc

Name:

Viral protein

Title:

The structure of bluetongue virus vp4 reveals a multifunctional RNA- capping production-line

Structure:

Vp4 core protein. Chain: a. Synonym: bluetongue virus vp4. Engineered: yes

Source:

Bluetongue virus 10 (isolate usa). Organism_taxid: 10900. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

3.00Å R-factor: 0.258 R-free: 0.333

Authors:

G.Sutton,J.M.Grimes,D.I.Stuart,P.Roy

Key ref:

G.Sutton et al. (2007). Bluetongue virus VP4 is an RNA-capping assembly line. Nat Struct Biol, 14, 449-451. PubMed id: 17417654 DOI: 10.1038/nsmb1225

Date:

21-Feb-07 Release date: 10-Apr-07

Protein chain

P07132  (VP4_BTV10) -  Core protein VP4 from Bluetongue virus 10 (isolate USA)

Seq: 644 a.a.

Struc: 613 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1038/nsmb1225

Nat Struct Biol 14:449-451 (2007)

PubMed id: 17417654

Bluetongue virus VP4 is an RNA-capping assembly line.

G.Sutton, J.M.Grimes, D.I.Stuart, P.Roy.

ABSTRACT

Eukaryotic organisms cap the 5' ends of their messenger RNAs by a series of four chemical reactions. Some viruses achieve this using a single molecule; the crystal structure of such an enzyme from bluetongue virus reveals an elongated modular architecture that provides a scaffold for an assemblage of active sites, two contributed by a domain of novel structure.

Selected figure(s)

Figure 1.
(a) Reaction mechanism for a type 1 cap. (b) VP4 domain organization (domain names defined in text). Poorly ordered loops are gray. Contact areas between domains are indicated. (c) VP4 colored by domain, with active sites and ligands colored as in a; view rotated 120° from b. AdoHcy in N7MTase and 2'OMTase are azure and orange, respectively. Cap analog is yellow. (d) Active sites mapped onto electrostatic surface representation of VP4 (colored as in c), and VP4 within BTV core.

Figure 2.
(a) Left, 2'OMT domain colored from blue (N terminus) to red (C terminus). Poorly ordered loop is gray. AdoHcy (magenta) and G5'ppp5'G (blue) substrates are shown. Right, 2'OMTase active sites of VP4 (purple) and VP39 (cyan). Carbon atoms and bonds of significant residues are colored gray. VP4 ligands colored as at left; VP39 AdoHcy and m^7G5'ppp5'G are marine and orange, respectively. Red and yellow spheres represent cap analog 2'-O and AdoHcy sulfur, respectively. (b) N7MT domain and AdoHcy, as in a. Right, stereo view of N7MTase active sites of VP4 and Ecm1. Carbon atoms and bonds of VP4 are gray, with guanine bases purple and AdoHcy pink. Ecm1 is in olive, with cap analog orange (blue sphere, N7) and AdoHcy lime (yellow spheres, sulfurs).

The above figures are reprinted by permission from Macmillan Publishers Ltd: Nat Struct Biol (2007, 14, 449-451) copyright 2007.

Figures were selected by an automated process.