PDBsum entry 2h2k

Metal binding protein

PDB id

2h2k

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Contents

			Protein chains

					93 a.a. *

			Metals

					_CA ×4

			Waters ×120

* Residue conservation analysis

PDB id:

2h2k

Links

Name:

Metal binding protein

Title:

Crystal structure analysis of human s100a13

Structure:

Protein s100-a13. Chain: a, b. Synonym: s100 calcium-binding protein a13. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.00Å

R-factor:

0.213

R-free:

0.249

Authors:

M.Li,P.F.Zhang,J.P.Zhang,W.R.Chang

Key ref:

M.Li et al. (2007). Crystal structure study on human S100A13 at 2.0 A resolution. Biochem Biophys Res Commun, 356, 616-621. PubMed id: 17374362

Date:

19-May-06

Release date:

22-May-07

PROCHECK

	Headers

	References

Protein chains

Q99584 (S10AD_HUMAN) - Protein S100-A13 from Homo sapiens

Seq: Struc:					98 a.a. 93 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

	Biochem Biophys Res Commun 356:616-621 (2007)
PubMed id: 17374362

Crystal structure study on human S100A13 at 2.0 A resolution.
M.Li, P.F.Zhang, X.W.Pan, W.R.Chang.

ABSTRACT

The S100 protein family is the largest group of calcium-binding protein families, which consists of at least 25 members. S100A13, which is widely expressed in a variety of tissues, is a unique member of the S100 protein family. Previous reports showed that S100A13 might be involved in the stress-induced release of some signal peptide-less proteins (such as FGF-1 and IL-1alpha) and also associated with inflammatory functions. It was also reported that S100A13 is a new angiogenesis marker. Here we report the crystal structure of the Ca(2+)-bound form of S100A13 at 2.0 A resolution. S100A13 is a homodimer with four EF-hand motifs in an asymmetric unit, displaying a folding pattern similar to other S100 members. However, S100A13 has the unique structural feature with all alpha-helices being amphiphilic, which was not found in other members of S100s. We propose that this characteristic structure of S100A13 might be related to its ability to mediate the release of FGF-1 and IL-1alpha.

Literature references that cite this PDB file's key reference

PubMed id

Reference

19890475

N.T.Wright, B.R.Cannon, D.B.Zimmer, and D.J.Weber (2009).
S100A1: Structure, Function, and Therapeutic Potential.

Curr Chem Biol, 3, 138-145.

18259052

F.L.Imai, K.Nagata, N.Yonezawa, M.Nakano, and M.Tanokura (2008).
Structure of calcium-bound human S100A13 at pH 7.5 at 1.8 A resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 70-76.

PDB code:

2egd

17786931

I.Prudovsky, F.Tarantini, M.Landriscina, D.Neivandt, R.Soldi, A.Kirov, D.Small, K.M.Kathir, D.Rajalingam, and T.K.Kumar (2008).
Secretion without Golgi.

J Cell Biochem, 103, 1327-1343.

18410126

V.N.Malashkevich, K.M.Varney, S.C.Garrett, P.T.Wilder, D.Knight, T.H.Charpentier, U.A.Ramagopal, S.C.Almo, D.J.Weber, and A.R.Bresnick (2008).
Structure of Ca2+-bound S100A4 and its interaction with peptides derived from nonmuscle myosin-IIA.

Biochemistry, 47, 5111-5126.

PDB code:

2q91

17991455

K.M.Kathir, K.Ibrahim, D.Rajalingam, I.Prudovsky, C.Yu, and T.K.Kumar (2007).
S100A13-lipid interactions-role in the non-classical release of the acidic fibroblast growth factor.

Biochim Biophys Acta, 1768, 3080-3089.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.