PDBsum entry 2gx0

Luminescent protein

PDB id

2gx0

Loading ...

Contents

			Protein chains

					218 a.a. *

			Waters ×610

* Residue conservation analysis

PDB id:

2gx0

Links

Name:

Luminescent protein

Title:

Crystal structural and functional analysis of gfp-like fluorescent protein

Structure:

Fluorescent protein dronpa. Chain: a, b, c, d. Fragment: green fluorescent protein. Synonym: gfp-like fluorescent protein. Engineered: yes

Source:

Echinophyllia sp. Sc22. Coral. Organism_taxid: 301887. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.90Å

R-factor:

0.178

R-free:

0.211

Authors:

K.Y.Hwang,K.-H.Nam,S.-Y.Park,K.Sugiyama

Key ref:

K.H.Nam et al. (2007). Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S. Biochem Biophys Res Commun, 354, 962-967. PubMed id: 17276392

Date:

08-May-06

Release date:

08-May-07

PROCHECK

	Headers

	References

Protein chains

Q5TLG6 (Q5TLG6_9CNID) - Fluorescent protein Dronpa from Echinophyllia sp. SC22

Seq: Struc:					224 a.a. 218 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

	Biochem Biophys Res Commun 354:962-967 (2007)
PubMed id: 17276392

Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S.
K.H.Nam, O.Y.Kwon, K.Sugiyama, W.H.Lee, Y.K.Kim, H.K.Song, E.E.Kim, S.Y.Park, H.Jeon, K.Y.Hwang.

ABSTRACT

The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking. Recently, Ando and his colleagues developed a new green fluorescent protein Dronpa, which possesses the unique photochromic property of being photoswitchable in a non-destructive manner. To better understand this mechanism, we determined the crystal structures of a new GFP Dronpa and its mutant C62S, at 1.9 Angstroms and 1.8 Angstroms, respectively. Determination of the structures demonstrates that a unique hydrogen-bonding network and the sulfur atom of the chromophore are critical to the photoswitching property of Dronpa. Reversible photoswitching was lost in cells expressing the Dronpa-C62S upon repetitive irradiation compared to the native protein. Structural and mutational analyses reveal the chemical basis for the functional properties of photoswitchable fluorescent proteins and provide the basis for subsequent coherent engineering of this subfamily of Dronpa homologs.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21052778

H.Mizuno, T.K.Mal, M.Wälchli, T.Fukano, M.Ikura, and A.Miyawaki (2010).
Molecular basis of photochromism of a fluorescent protein revealed by direct 13C detection under laser illumination.

J Biomol NMR, 48, 237-246.

20373040

Y.R.Lee, J.H.Park, S.H.Hahm, L.W.Kang, J.H.Chung, K.H.Nam, K.Y.Hwang, I.C.Kwon, and Y.S.Han (2010).
Development of bimolecular fluorescence complementation using Dronpa for visualization of protein-protein interactions in cells.

Mol Imaging Biol, 12, 468-478.

18574155

H.Mizuno, T.K.Mal, M.Wälchli, A.Kikuchi, T.Fukano, R.Ando, J.Jeyakanthan, J.Taka, Y.Shiro, M.Ikura, and A.Miyawaki (2008).
Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.

Proc Natl Acad Sci U S A, 105, 9227-9232.

PDB codes:

2z1o 2z6x 2z6y 2z6z

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.