PDBsum entry 2gim

Electron transport

PDB id: 2gim

Contents

Protein chains

106 a.a. *

Metals

_CU ×2

Waters ×315

* Residue conservation analysis

PDB id:

2gim

Name:

Electron transport

Title:

1.6 angstrom structure of plastocyanin from anabaena variabilis

Structure:

Plastocyanin. Chain: a, c. Engineered: yes

Source:

Anabaena variabilis. Organism_taxid: 1172. Gene: pete. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å R-factor: 0.211 R-free: 0.260

Authors:

L.Schmidt,P.Harris,H.E.M.Christensen

Key ref:

L.Schmidt et al. (2006). Structure of plastocyanin from the cyanobacterium Anabaena variabilis. Acta Crystallogr D Biol Crystallogr, 62, 1022-1029. PubMed id: 16929103 DOI: 10.1107/S0907444906023638

Date:

29-Mar-06 Release date: 11-Apr-06

Protein chains

P0C178  (PLAS_ANAVA) -  Plastocyanin from Anabaena variabilis

Seq: 105 a.a.

Struc: 106 a.a.

DOI no: 10.1107/S0907444906023638

Acta Crystallogr D Biol Crystallogr 62:1022-1029 (2006)

PubMed id: 16929103

Structure of plastocyanin from the cyanobacterium Anabaena variabilis.

L.Schmidt, H.E.Christensen, P.Harris.

ABSTRACT

Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.

Selected figure(s)

Figure 2.
Figure 2 Superimposed structures of A. variabilis plastocyanin (red and cyan) and poplar plastocyanin (green and grey; PDB code 1plc ; Guss et al., 1992[Guss, J. M., Bartunik, H. D. & Freeman, H. C. (1992). Acta Cryst. B48, 790-811.]). The structures are represented by the C^ atoms and the Cu ions. 58-61 refer to the residues Ser58-Asp61 in poplar plastocyanin. The figure was prepared using CCP4mg (Potterton et al., 2002[Potterton, E., McNicholas, S., Krissinel, E., Cowtan, K. & Noble, M. (2002). Acta Cryst. D58, 1955-1957.]).

Figure 5.
Figure 5 2F[obs] - F[calc] [A]-weighted electron-density map showing the hydrogen-bonding networks of the two conformations of His61 in A. variabilis chains A (left) and C (right). The higher occupied (occupancy 0.55 in chain A and 0.65 in chain C) conformations are shown in green (C-atom colour) and the lower occupied (0.45 in chain A and 0.35 in chain C) are shown in light blue. This figure was produced using PyMOL (DeLano, 2002[DeLano, W. L. (2002). The PyMOL Molecular Visualization System. DeLano Scientific, San Carlos, CA, USA. http://www.pymol.org .]).

The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2006, 62, 1022-1029) copyright 2006.

Figures were selected by the author.