PDBsum entry 2g4f

Hydrolase

PDB id: 2g4f

Contents

Protein chains

302 a.a. *

Waters ×118

* Residue conservation analysis

PDB id:

2g4f

Name:

Hydrolase

Title:

Structure of s.Olivaceoviridis xylanase q88a/r275a mutant

Structure:

Hydrolase. Chain: a, b. Fragment: catalytic domain, residues 1-302. Synonym: xylanase. Engineered: yes. Mutation: yes

Source:

Streptomyces olivaceoviridis. Organism_taxid: 1921. Strain: e-86. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.65Å R-factor: 0.169 R-free: 0.231

Authors:

S.Diertavitian,S.Kaneko,Z.Fujimoto,A.Kuno,E.Johansson,L.Lo Leggio

Key ref:

S.Diertavitian et al. Variants of s.Olivaceoviridis xylanase. To be published, .

Date:

22-Feb-06 Release date: 06-Mar-07

Protein chains

Q7SI98  (Q7SI98_STROI) -  Beta-xylanase from Streptomyces olivaceoviridis

Seq: 436 a.a.

Struc: 302 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.8 - endo-1,4-beta-xylanase.
• Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.