PDBsum entry 2fv4

Structural protein, protein binding

PDB id: 2fv4

Contents

Protein chains

89 a.a.

58 a.a. *

* Residue conservation analysis

PDB id:

2fv4

Name:

Structural protein, protein binding

Title:

Nmr solution structure of the yeast kinetochore spc24/spc25 globular domain

Structure:

Hypothetical 25.2 kda protein in afg3-seb2 intergenic region. Chain: a. Fragment: spc25p globular domain. Engineered: yes. Hypothetical 24.6 kda protein in ilv2-ade17 intergenic region. Chain: b. Fragment: spc24p globular domain.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: yer018c. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_cell_line: rosetta. Gene: ymr117c, ym9718.16c.

NMR struc:

20 models

Authors:

J.R.Schnell,J.J.Chou

Key ref:

R.R.Wei et al. (2006). Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain. Structure, 14, 1003-1009. PubMed id: 16765893 DOI: 10.1016/j.str.2006.04.007

Date:

29-Jan-06 Release date: 13-Jun-06

Protein chain

P40014  (SPC25_YEAST) -  Kinetochore protein SPC25 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 221 a.a.

Struc: 89 a.a.

Protein chain

Q04477  (SPC24_YEAST) -  Kinetochore protein SPC24 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 213 a.a.

Struc: 58 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.?

DOI no: 10.1016/j.str.2006.04.007

Structure 14:1003-1009 (2006)

PubMed id: 16765893

Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain.

R.R.Wei, J.R.Schnell, N.A.Larsen, P.K.Sorger, J.J.Chou, S.C.Harrison.

ABSTRACT

The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.

Selected figure(s)

Figure 1.
Figure 1. Schematic Diagram of the Ndc80 Complex
MT, microtubule; CEN, centromere. Each subunit is represented by an oval (the globular domain) and a stick (the coiled-coil region). The coiled-coils of Ndc80p/Nuf2p and Spc24p/Spc25p form the shaft. The globular domains of Ndc80p/Nuf2p form the “outer” head, which faces the microtubule; the globular domains of the Spc24p/Spc25p, the “inner” head, which faces the centromere.

Figure 2.
Figure 2. Overview of the NMR and Crystal Structures of Spc24G/Spc25G
(A) Superposition of the backbones of the solution NMR structure (light blue) and crystal structure (gold). The dotted lines represent unstructured residues 138–154 of Spc24G and 128–132 of Spc25G. The N and C termini of Spc24G and Spc25G are labeled with “ 24N,” “24C,” “25N,” and “25C,” respectively.
(B) Ribbon diagram of the crystal structure in the same view as in (A). The α helices and β strands are orange and yellow in Spc24G and green and blue in Spc25G, respectively.
(C) Folding diagram of Spc24G and Spc25G. The secondary structures are colored as in (B). The N and C termini are labeled with “N” and “C,” respectively.

The above figures are reprinted by permission from Cell Press: Structure (2006, 14, 1003-1009) copyright 2006.

Figures were selected by an automated process.