PDBsum entry 2fkp

Isomerase

PDB id: 2fkp

Contents

Protein chains

360 a.a. *

Waters ×891

* Residue conservation analysis

PDB id:

2fkp

Name:

Isomerase

Title:

The mutant g127c-t313c of deinococcus radiodurans n-acylamino acid racemase

Structure:

N-acylamino acid racemase. Chain: a, b, c, d. Engineered: yes. Mutation: yes

Source:

Deinococcus radiodurans. Organism_taxid: 1299. Strain: ccrc 12827. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Octamer (from PDB file)

Resolution:

2.00Å R-factor: 0.184 R-free: 0.227

Authors:

W.C.Wang,W.C.Chiu

Key ref:

W.C.Wang and w.c.chiu Enhanced thermoactivity in covalently cross-Linked n-Carbamoyl d-Amino acid amidohydrolase but not in n-Acylamino acid racemase that has induced fit movements upon substrate binding. To be published, .

Date:

05-Jan-06 Release date: 24-Jan-06

Supersedes:

2bah

Protein chains

Q9RYA6  (Q9RYA6_DEIRA) -  N-succinylamino acid racemase from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)

Seq: 375 a.a.

Struc: 360 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.2.1.113 - o-succinylbenzoate synthase.
• Reaction: (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O
• Cofactor: Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site