PDBsum entry 2ff0

Hormone/growth factor/DNA

PDB id: 2ff0

Contents

Protein chain

102 a.a. *

DNA/RNA

Metals

_ZN ×2

* Residue conservation analysis

PDB id:

2ff0

Name:

Hormone/growth factor/DNA

Title:

Solution structure of steroidogenic factor 1 DNA binding domain bound to its target sequence in the inhibin alpha-subunit promoter

Structure:

Ggctcagggccacag. Chain: b. Fragment: inhibin alpha-subunit promoter. Engineered: yes. Ctgtggccctgagcc. Chain: c. Fragment: inhibin alpha-subunit promoter. Engineered: yes. Steroidogenic factor 1.

Source:

Synthetic: yes. Other_details: the DNA sequence was synthesized via automated methods. Mus musculus. House mouse. Organism_taxid: 10090. Gene: nr5a1, ftzf1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

NMR struc:

16 models

Authors:

T.H.Little,Y.Zhang,C.K.Matulis,J.Weck,Z.Zhang,A.Ramachandran, K.E.Mayo,I.Radhakrishnan

Key ref:

T.H.Little et al. (2006). Sequence-specific deoxyribonucleic acid (DNA) recognition by steroidogenic factor 1: a helix at the carboxy terminus of the DNA binding domain is necessary for complex stability. Mol Endocrinol, 20, 831-843. PubMed id: 16339274

Date:

17-Dec-05 Release date: 11-Apr-06

Protein chain

P33242  (STF1_MOUSE) -  Steroidogenic factor 1 from Mus musculus

Seq: 462 a.a.

Struc: 102 a.a.

DNA/RNA chains

G-G-C-T-C-A-G-G-G-C-C-A-C-A-G 15 bases

C-T-G-T-G-G-C-C-C-T-G-A-G-C-C 15 bases

Enzyme reactions

• Enzyme class: E.C.?

Mol Endocrinol 20:831-843 (2006)

PubMed id: 16339274

Sequence-specific deoxyribonucleic acid (DNA) recognition by steroidogenic factor 1: a helix at the carboxy terminus of the DNA binding domain is necessary for complex stability.

T.H.Little, Y.Zhang, C.K.Matulis, J.Weck, Z.Zhang, A.Ramachandran, K.E.Mayo, I.Radhakrishnan.

ABSTRACT

Steroidogenic factor 1 (SF1) is a member of the NR5A subfamily of nuclear hormone receptors and is considered a master regulator of reproduction because it regulates a number of genes encoding reproductive hormones and enzymes involved in steroid hormone biosynthesis. Like other NR5A members, SF1 harbors a highly conserved approximately 30-residue segment called the FTZ-F1 box C-terminal to the core DNA binding domain (DBD) common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-alpha gene that encodes a subunit of a reproductive hormone. SF1 forms a specific complex with the DNA through a bipartite motif binding to the major and minor grooves through the core DBD and the N-terminal segment of the FTZ-F1 box, respectively, in a manner previously described for two other monomeric receptors, nerve growth factor-induced-B and estrogen-related receptor 2. However, unlike these receptors, SF1 harbors a helix in the C-terminal segment of the FTZ-F1 box that interacts with both the core DBD and DNA and serves as an important determinant of stability of the complex. We propose that the FTZ-F1 helix along with the core DBD serves as a platform for interactions with coactivators and other DNA-bound factors in the vicinity.