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PDBsum entry 2fd2
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Electron transfer(iron-sulfur protein)
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PDB id
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2fd2
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Contents |
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* Residue conservation analysis
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J Biol Chem
266:21558-21562
(1991)
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PubMed id:
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Crystallographic analysis of two site-directed mutants of Azotobacter vinelandii ferredoxin.
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J.Soman,
S.Iismaa,
C.D.Stout.
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ABSTRACT
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The crystal structure of the C24A mutant of Azotobacter vinelandii 7Fe
ferredoxin (FdI) has been solved and refined at 2.0-A resolution. The structure
is isomorphous to native FdI except at the site of mutation where A24 moves
toward the [4Fe-4S] cluster. In spite of this inefficient packing results: three
of five van der Waals contacts from the S gamma of C24 in native FdI are lost
and the remaining two become longer. Consequently, the [4Fe-4S] cluster is
either disordered or has a higher temperature factor (B factor) compared to the
rest of the C24A FdI molecule. In addition, the entire C24A FdI structure has a
higher overall B factor than native FdI. Therefore, in comparison to native FdI,
the C24A mutant is isomorphous but exhibits large differences in B factor,
especially at the [4Fe-4S] cluster. In contrast, the C20A FdI structure (Martin,
A. G., Burgess, B. K., Stout, C. D., Cash, V. L., Dean, D. R., Jensen, G. M.,
and Stephens, P. J. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 598-602), which
contains large structural rearrangements in the vicinity of the [4Fe-4S]
cluster, exhibits essentially no change in B factor. The conformational change
observed at residue 24 is similar in both C24A and C20A FdI structures. The
solvent accessibility of the Fe atoms in the [3Fe-4S] and [4Fe-4S] clusters is
similar in C24A, C20A, and native FdI.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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E.Saridakis,
P.Giastas,
G.Efthymiou,
V.Thoma,
J.M.Moulis,
P.Kyritsis,
and
I.M.Mavridis
(2009).
Insight into the protein and solvent contributions to the reduction potentials of [4Fe-4S]2+/+ clusters: crystal structures of the Allochromatium vinosum ferredoxin variants C57A and V13G and the homologous Escherichia coli ferredoxin.
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J Biol Inorg Chem,
14,
783-799.
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PDB codes:
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B.W.Beck,
Q.Xie,
and
T.Ichiye
(2001).
Sequence determination of reduction potentials by cysteinyl hydrogen bonds and peptide pipoles in [4Fe-4S] ferredoxins.
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Biophys J,
81,
601-613.
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J.A.Tainer,
V.A.Roberts,
and
E.D.Getzoff
(1992).
Protein metal-binding sites.
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Curr Opin Biotechnol,
3,
378-387.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
