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PDBsum entry 2f2h
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Contents |
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* Residue conservation analysis
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PDB id:
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Hydrolase
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Title:
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Structure of the yici thiosugar michaelis complex
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Structure:
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Putative family 31 glucosidase yici. Chain: a, b, c, d, e, f. Engineered: yes
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Source:
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Escherichia coli. Organism_taxid: 562. Gene: yici. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Biol. unit:
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Hexamer (from
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Resolution:
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1.95Å
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R-factor:
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0.182
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R-free:
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0.225
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Authors:
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Y.-W.Kim,A.L.Lovering,N.C.J.Strynadka,S.G.Withers
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Key ref:
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Y.W.Kim
et al.
(2006).
Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex.
J Am Chem Soc,
128,
2202-2203.
PubMed id:
DOI:
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Date:
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16-Nov-05
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Release date:
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28-Feb-06
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PROCHECK
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Headers
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References
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P31434
(XYLS_ECOLI) -
Alpha-xylosidase from Escherichia coli (strain K12)
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Seq:
Struc:
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772 a.a.
773 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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J Am Chem Soc
128:2202-2203
(2006)
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PubMed id:
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Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex.
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Y.W.Kim,
A.L.Lovering,
H.Chen,
T.Kantner,
L.P.McIntosh,
N.C.Strynadka,
S.G.Withers.
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ABSTRACT
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For the first time, the thioglycoligase strategy has been successfully applied
to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31
glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from
Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl
fluorides and deoxythioglycosides as donors and acceptors, respectively, in
yields up to 86%. In addition, we describe the Michaelis complex of YicI using
one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss
the structural insights this yields into the specificity and mechanism of family
31 alpha-glycosidases and the molecular basis of an associated genetic disease.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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Y.W.Kim,
R.Zhang,
H.Chen,
and
S.G.Withers
(2010).
O-glycoligases, a new category of glycoside bond-forming mutant glycosidases, catalyse facile syntheses of isoprimeverosides.
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Chem Commun (Camb),
46,
8725-8727.
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A.Homann,
and
J.Seibel
(2009).
Chemo-enzymatic synthesis and functional analysis of natural and modified glycostructures.
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Nat Prod Rep,
26,
1555-1571.
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L.X.Wang,
and
W.Huang
(2009).
Enzymatic transglycosylation for glycoconjugate synthesis.
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Curr Opin Chem Biol,
13,
592-600.
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M.S.Kang,
M.Okuyama,
K.Yaoi,
Y.Mitsuishi,
Y.M.Kim,
H.Mori,
D.Kim,
and
A.Kimura
(2007).
Aglycone specificity of Escherichia coli alpha-xylosidase investigated by transxylosylation.
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FEBS J,
274,
6074-6084.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
