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PDBsum entry 2exr

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protein ligands links
Oxidoreductase PDB id
2exr

 

 

 

 

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Contents
Protein chain
491 a.a. *
Ligands
FAD
Waters ×633
* Residue conservation analysis
PDB id:
2exr
Name: Oxidoreductase
Title: X-ray structure of cytokinin oxidase/dehydrogenase (ckx) from arabidopsis thaliana at5g21482
Structure: Cytokinin dehydrogenase 7. Chain: a. Synonym: cytokinin oxidase 7, cko7, atckx7, atckx5. Engineered: yes
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Gene: ckx7, ckx5. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.70Å     R-factor:   0.173     R-free:   0.214
Authors: G.E.Wesenberg,G.N.Phillips Jr.,B.W.Han,E.Bitto,C.A.Bingman,E.Bae, Center For Eukaryotic Structural Genomics (Cesg)
Key ref:
E.Bae et al. (2008). Crystal structure of Arabidopsis thaliana cytokinin dehydrogenase. Proteins, 70, 303-306. PubMed id: 17886275 DOI: 10.1002/prot.21678
Date:
08-Nov-05     Release date:   29-Nov-05    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9FUJ1  (CKX7_ARATH) -  Cytokinin dehydrogenase 7 from Arabidopsis thaliana
Seq:
Struc:
 
Seq:
Struc:
524 a.a.
491 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.1.5.99.12  - cytokinin dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: N6-dimethylallyladenine + A + H2O = 3-methyl-2-butenal + adenine + AH2
N(6)-dimethylallyladenine
+
+ H2O
= 3-methyl-2-butenal
+ adenine
+ AH2
      Cofactor: FAD
FAD
Bound ligand (Het Group name = FAD) corresponds exactly
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
DOI no: 10.1002/prot.21678 Proteins 70:303-306 (2008)
PubMed id: 17886275  
 
 
Crystal structure of Arabidopsis thaliana cytokinin dehydrogenase.
E.Bae, C.A.Bingman, E.Bitto, D.J.Aceti, G.N.Phillips.
 
  ABSTRACT  
 
No abstract given.

 
  Selected figure(s)  
 
Figure 1.
Figure 1. Crystal structure of AtCKX7. (A) Ribbon diagram of AtCKX7. The FAD-binding and the substrate-binding domains are shown in cyan and red, respectively. The FAD molecule is represented as a stick model in yellow. (B) Covalent bond between His96 and the isoalloxazine ring of the FAD molecule. The FAD is shown in yellow and the His residue is colored by atom types (carbon: green; oxygen: red; nitrogen: blue). The 2F[obs] - F[calc] map is contoured at 1.5 .
 
  The above figure is reprinted by permission from John Wiley & Sons, Inc.: Proteins (2008, 70, 303-306) copyright 2008.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
19740698 T.Werner, and T.Schmülling (2009).
Cytokinin action in plant development.
  Curr Opin Plant Biol, 12, 527-538.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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