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PDBsum entry 2evs
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Lipid transport
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PDB id
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2evs
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Contents |
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* Residue conservation analysis
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Plos Biol
4:e362
(2006)
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PubMed id:
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The liganding of glycolipid transfer protein is controlled by glycolipid acyl structure.
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L.Malinina,
M.L.Malakhova,
A.T.Kanack,
M.Lu,
R.Abagyan,
R.E.Brown,
D.J.Patel.
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ABSTRACT
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Glycosphingolipids (GSLs) play major roles in cellular growth and development.
Mammalian glycolipid transfer proteins (GLTPs) are potential regulators of cell
processes mediated by GSLs and display a unique architecture among lipid
binding/transfer proteins. The GLTP fold represents a novel membrane
targeting/interaction domain among peripheral proteins. Here we report crystal
structures of human GLTP bound to GSLs of diverse acyl chain length,
unsaturation, and sugar composition. Structural comparisons show a highly
conserved anchoring of galactosyl- and lactosyl-amide headgroups by the GLTP
recognition center. By contrast, acyl chain chemical structure and occupancy of
the hydrophobic tunnel dictate partitioning between sphingosine-in and
newly-observed sphingosine-out ligand-binding modes. The structural insights,
combined with computed interaction propensity distributions, suggest a concerted
sequence of events mediated by GLTP conformational changes during GSL transfer
to and/or from membranes, as well as during GSL presentation and/or transfer to
other proteins.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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D.K.Breslow,
and
J.S.Weissman
(2010).
Membranes in balance: mechanisms of sphingolipid homeostasis.
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Mol Cell,
40,
267-279.
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P.Haimi,
M.Hermansson,
K.C.Batchu,
J.A.Virtanen,
and
P.Somerharju
(2010).
Substrate efflux propensity plays a key role in the specificity of secretory A-type phospholipases.
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J Biol Chem,
285,
751-760.
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X.Zhai,
M.L.Malakhova,
H.M.Pike,
L.M.Benson,
H.R.Bergen,
I.P.Sugár,
L.Malinina,
D.J.Patel,
and
R.E.Brown
(2009).
Glycolipid Acquisition by Human Glycolipid Transfer Protein Dramatically Alters Intrinsic Tryptophan Fluorescence: INSIGHTS INTO GLYCOLIPID BINDING AFFINITY.
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J Biol Chem,
284,
13620-13628.
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G.D'Angelo,
M.Vicinanza,
and
M.A.De Matteis
(2008).
Lipid-transfer proteins in biosynthetic pathways.
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Curr Opin Cell Biol,
20,
360-370.
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G.West,
L.Viitanen,
C.Alm,
P.Mattjus,
T.A.Salminen,
and
J.Edqvist
(2008).
Identification of a glycosphingolipid transfer protein GLTP1 in Arabidopsis thaliana.
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FEBS J,
275,
3421-3437.
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N.H.Petersen,
L.V.McKinney,
H.Pike,
D.Hofius,
A.Zakaria,
P.Brodersen,
M.Petersen,
R.E.Brown,
and
J.Mundy
(2008).
Human GLTP and mutant forms of ACD11 suppress cell death in the Arabidopsis acd11 mutant.
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FEBS J,
275,
4378-4388.
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X.Zou,
T.Chung,
X.Lin,
M.L.Malakhova,
H.M.Pike,
and
R.E.Brown
(2008).
Human glycolipid transfer protein (GLTP) genes: organization, transcriptional status and evolution.
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BMC Genomics,
9,
72.
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R.E.Brown,
and
P.Mattjus
(2007).
Glycolipid transfer proteins.
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Biochim Biophys Acta,
1771,
746-760.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
