PDBsum entry 2ei3

Oxidoreductase

PDB id: 2ei3

Contents

Protein chain

298 a.a. *

Ligands

BPY ×3

GOL ×2

Metals

_MG

FE2

Waters ×202

* Residue conservation analysis

PDB id:

2ei3

Name:

Oxidoreductase

Title:

Anaerobic crystal structure analysis of the 1,2-dihydroxynaphthalene dioxygenase from pseudomonas sp. Strain c18 complexes with 2,3- dihydroxybiphenyl

Structure:

1,2-dihydroxynaphthalene dioxygenase. Chain: a. Engineered: yes

Source:

Pseudomonas sp.. Organism_taxid: 69011. Strain: c18. Gene: doxg. Expressed in: pseudomonas putida. Expression_system_taxid: 303.

Resolution:

1.90Å R-factor: 0.182 R-free: 0.206

Authors:

D.B.Neau,M.S.Kelker,C.L.Colbert,J.T.Bolin

Key ref:

D.B.Neau et al. Structural explanation for success and failure in the enzymatic ring-Cleavage of 3,4 dihydroxybiphenyl and related pcb metabolites. To be published, .

Date:

10-Mar-07 Release date: 19-Feb-08

Protein chain

P0A108  (NAHC_PSEU8) -  1,2-dihydroxynaphthalene dioxygenase from Pseudomonas sp. (strain C18)

Seq: 302 a.a.

Struc: 298 a.a.

Enzyme reactions

• Enzyme class: E.C.1.13.11.56 - 1,2-dihydroxynaphthalene dioxygenase.
• Reaction: naphthalene-1,2-diol + O2 = 2-hydroxychromene-2-carboxylate + H⁺

naphthalene-1,2-diol (Bound ligand (Het Group name = BPY) matches with 85.71% similarity) + O2 = 2-hydroxychromene-2-carboxylate + H(+)

• Cofactor: Fe cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site