PDBsum entry 2eb8

Oxygen storage/transport

PDB id

2eb8

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Contents

			Protein chain

					154 a.a. *

			Ligands

					PO4 ×5


					CUP

			Waters ×136

* Residue conservation analysis

PDB id:

2eb8

Links

Name:

Oxygen storage/transport

Title:

Crystal structure of cu(ii)(sal-phe)/apo-myoglobin

Structure:

Myoglobin. Chain: a. Engineered: yes

Source:

Physeter catodon. Sperm whale. Organism_taxid: 9755. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.65Å

R-factor:

0.205

R-free:

0.246

Authors:

S.Abe,S.Okazaki,T.Ueno,T.Hikage,A.Suzuki,T.Yamane,Y.Watanabe

Key ref:

S.Abe et al. (2007). Design and structure analysis of artificial metalloproteins: selective coordination of His64 to copper complexes with square-planar structure in the apo-myoglobin scaffold. Inorg Chem, 46, 5137-5139. PubMed id: 17523632

Date:

07-Feb-07

Release date:

03-Jul-07

PROCHECK

	Headers

	References

Protein chain

P02185 (MYG_PHYMC) - Myoglobin from Physeter macrocephalus

Seq: Struc:					154 a.a. 154 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

	Inorg Chem 46:5137-5139 (2007)
PubMed id: 17523632

Design and structure analysis of artificial metalloproteins: selective coordination of His64 to copper complexes with square-planar structure in the apo-myoglobin scaffold.
S.Abe, T.Ueno, P.A.Reddy, S.Okazaki, T.Hikage, A.Suzuki, T.Yamane, H.Nakajima, Y.Watanabe.

ABSTRACT

apo-Myoglobin (apo-Mb) was reconstituted with three copper complexes: CuII(Sal-Phe) (1; Sal-Phe = N-salicylidene-L-phenylalanato), CuII(Sal-Leu) (2; Sal-Leu = N-salicylidene-L-leucinato), and CuII(Sal-Ala) (3; Sal-Ala = N-salicylidene-L-alanato). The crystal structures of 1.apo-Mb (1.65 Angstrom resolution) and 2.apo-Mb (1.8 Angstrom resolution) show that the coordination geometry around the CuII atom in apo-Mb is distorted square-planar with tridentate Sal-X and a Nepsilon atom of His64 in the apo-Mb cavity and the plane of these copper complexes is perpendicular to that of heme. These results suggest that the apo-Mb cavity can hold metal complexes with various coordination geometries.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20820465

A.Dalla Cort, P.De Bernardin, G.Forte, and F.Y.Mihan (2010).
Metal-salophen-based receptors for anions.

Chem Soc Rev, 39, 3863-3874.

19057765

J.Niemeyer, S.Abe, T.Hikage, T.Ueno, G.Erker, and Y.Watanabe (2008).
Noncovalent insertion of ferrocenes into the protein shell of apo-ferritin.

Chem Commun (Camb), (), 6519-6521.

18682861

M.R.Maurya, M.Kumar, A.Kumar, and J.Costa Pessoa (2008).
Oxidation of p-chlorotoluene and cyclohexene catalysed by polymer-anchored oxovanadium(IV) and copper(II) complexes of amino acid derived tridentate ligands.

Dalton Trans, (), 4220-4232.

18092096

N.Yokoi, T.Ueno, M.Unno, T.Matsui, M.Ikeda-Saito, and Y.Watanabe (2008).
Ligand design for the improvement of stability of metal complex.protein hybrids.

Chem Commun (Camb), (), 229-231.

PDB code:

2z68

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.