PDBsum entry 2d8a

Oxidoreductase

PDB id: 2d8a

Contents

Protein chain

333 a.a. *

Ligands

NAD

Metals

_ZN ×5

Waters ×263

* Residue conservation analysis

PDB id:

2d8a

Name:

Oxidoreductase

Title:

Crystal structure of ph0655 from pyrococcus horikoshii ot3

Structure:

Probable l-threonine 3-dehydrogenase. Chain: a. Synonym: ph0655. Engineered: yes

Source:

Pyrococcus horikoshii. Organism_taxid: 70601. Strain: ot3. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Tetramer (from PDB file)

Resolution:

2.05Å R-factor: 0.204 R-free: 0.235

Authors:

Y.Asada,N.Kunishima,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

Y.Asada and n.kunishima Crystal structure of ph0655 from pyrococcus horikoshii ot3. To be published, .

Date:

02-Dec-05 Release date: 02-Jun-06

Protein chain

O58389  (TDH_PYRHO) -  L-threonine 3-dehydrogenase from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

Seq: 348 a.a.

Struc: 333 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.103 - L-threonine 3-dehydrogenase.
• Reaction: L-threonine + NAD⁺ = (2S)-2-amino-3-oxobutanoate + NADH + H⁺

L-threonine + NAD(+) (Bound ligand (Het Group name = NAD) corresponds exactly) = (2S)-2-amino-3-oxobutanoate + NADH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site