PDBsum entry 2d5y

Transferase

PDB id: 2d5y

Contents

Protein chain

396 a.a. *

Ligands

PLP

IVA

Waters ×162

* Residue conservation analysis

PDB id:

2d5y

Name:

Transferase

Title:

Aspartate aminotransferase mutant mc with isovaleric acid

Structure:

Aspartate aminotransferase. Chain: a. Synonym: transaminase a, aspat. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Strain: jm109. Gene: aspc. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PDB file)

Resolution:

1.98Å R-factor: 0.184 R-free: 0.216

Authors:

Y.Tanaka,N.Nakagawa,H.Tada,T.Yano,R.Masui,S.Kuramitsu

Key ref:

Y.Tanaka et al. The structures of aspartate aminotransferase with mutations of non-Active-Site residues. To be published, .

Date:

08-Nov-05 Release date: 14-Nov-06

Protein chain

P00509  (AAT_ECOLI) -  Aspartate aminotransferase from Escherichia coli (strain K12)

Seq: 396 a.a.

Struc: 396 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.6.1.1 - aspartate transaminase.
• Reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

L-aspartate (Bound ligand (Het Group name = IVA) matches with 60.00% similarity) + 2-oxoglutarate = oxaloacetate + L-glutamate

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site