PDBsum entry 2d4e

Oxidoreductase

PDB id: 2d4e

Contents

Protein chains

513 a.a. *

Ligands

GOL ×3

NAD ×2

Metals

_NA ×6

Waters ×1569

* Residue conservation analysis

PDB id:

2d4e

Name:

Oxidoreductase

Title:

Crystal structure of the hpcc from thermus thermophilus hb8

Structure:

5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase. Chain: a, b, c, d. Engineered: yes

Source:

Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Tetramer (from PQS)

Resolution:

2.10Å R-factor: 0.179 R-free: 0.212

Authors:

H.Mizutani,N.Kunishima,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

H.Mizutani and n.kunishima Crystal structure of the hpcc from thermus thermophilus hb8. To be published, .

Date:

18-Oct-05 Release date: 18-Apr-06

Protein chains

Q5SJP9  (Q5SJP9_THET8) -  5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenaseiheyensis HTE831] from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 515 a.a.

Struc: 513 a.a.

Enzyme reactions

• Enzyme class: E.C.1.2.1.60 - 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase.
• Reaction: 2-hydroxy-5-carboxymethylmuconate semialdehyde + NAD⁺ + H2O = (2E,4Z)- 5-hydroxypenta-2,4-diene-1,2,5-tricarboxylate + NADH + 2 H⁺

2-hydroxy-5-carboxymethylmuconate semialdehyde + NAD(+) + H2O (Bound ligand (Het Group name = NAD) corresponds exactly) = (2E,4Z)- 5-hydroxypenta-2,4-diene-1,2,5-tricarboxylate + NADH + 2 × H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site