PDBsum entry 2cik

Immune system/peptide

PDB id

2cik

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Contents

			Protein chains

					276 a.a. *


					99 a.a. *

			Ligands

					LYS-PRO-ILE-VAL- VAL-LEU-HIS-GLY- TYR


					GOL ×3

			Waters ×325

* Residue conservation analysis

PDB id:

2cik

Links

Name:

Immune system/peptide

Title:

Insights into crossreactivity in human allorecognition: the structure of hla-b35011 presenting an epitope derived from cytochrome p450.

Structure:

Hla class i histocompatibility antigen b-35 alpha chain. Chain: a. Fragment: residues 25-300. Synonym: hla-b3501, mhc class i antigen b 35, Hla class i histocompatibility antigen b3501 heavy chain, b350101, b35011, b35, b35, b3501. Engineered: yes. Beta-2-microglobulin. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: (de3)plyss. Synthetic: yes. Other_details: peptide epitope from human cytochrome p450 isoforms iic8, iic9, iic10 and iic18

Biol. unit:

Trimer (from PDB file)

Resolution:

1.75Å

R-factor:

0.198

R-free:

0.227

Authors:

C.S.Hourigan,M.Harkiolaki,N.A.Peterson,J.I.Bell,E.Y.Jones, C.A.O'Callaghan

Key ref:

C.S.Hourigan et al. (2006). The structure of the human allo-ligand HLA-B*3501 in complex with a cytochrome p450 peptide: steric hindrance influences TCR allo-recognition. Eur J Immunol, 36, 3288-3293. PubMed id: 17109469 DOI: 10.1002/eji.200636234

Date:

22-Mar-06

Release date:

25-Oct-06

PROCHECK

	Headers

	References

Protein chain

O19626 (O19626_HUMAN) - HLA-B35 (Fragment) from Homo sapiens

Seq: Struc:					322 a.a. 276 a.a.

Protein chain

P61769 (B2MG_HUMAN) - Beta-2-microglobulin from Homo sapiens

Seq: Struc:					119 a.a. 99 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

DOI no: 10.1002/eji.200636234	Eur J Immunol 36:3288-3293 (2006)
PubMed id: 17109469

The structure of the human allo-ligand HLA-B*3501 in complex with a cytochrome p450 peptide: steric hindrance influences TCR allo-recognition.
C.S.Hourigan, M.Harkiolaki, N.A.Peterson, J.I.Bell, E.Y.Jones, C.A.O'Callaghan.

ABSTRACT

Virus-specific T cell populations have been implicated in allo-recognition. The subdominant T cell receptor JL12 recognizes both HLA-B*0801 presenting the Epstein-Barr virus-derived peptide FLRGRAYGL and also HLA-B*3501 presenting the cytochrome p450 self peptide KPIVVLHGY. This cross-reactivity could promote the rejection of HLA-B*3501-positive cells in Epstein-Barr virus-exposed HLA-B*0801 recipients. LC13, the dominant TCR against the HLA-B*0801:FLRGRAYGL complex, fails to recognize HLA-B*3501:KPIVVLHGY. We report the 1.75-Angstrom resolution crystal structure of the human allo-ligand HLA-B*3501:KPIVVLHGY. Similarities between this structure and that of HLA-B*0801:FLRGRAYGL may facilitate cross-recognition by JL12. Moreover, the elevated peptide position in HLA-B*3501:KPIVVLHGY would provide steric hindrance to LC13, preventing it from interacting in the manner in which it interacts with HLA-B*0801:FLRGRAYGL. These findings are relevant to understanding the basis of T cell cross-reactivity in allo-recognition, optimal transplant donor-recipient matching and developing specific molecular inhibitors of allo-recognition.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20616031

S.Gras, L.Kedzierski, S.A.Valkenburg, K.Laurie, Y.C.Liu, J.T.Denholm, M.J.Richards, G.F.Rimmelzwaan, A.Kelso, P.C.Doherty, S.J.Turner, J.Rossjohn, and K.Kedzierska (2010).
Cross-reactive CD8+ T-cell immunity between the pandemic H1N1-2009 and H1N1-1918 influenza A viruses.

Proc Natl Acad Sci U S A, 107, 12599-12604.

PDB codes:

3lkn 3lko 3lkp 3lkq 3lkr 3lks

20347630

T.G.Tan, E.Mui, H.Cong, W.H.Witola, A.Montpetit, S.P.Muench, J.Sidney, J.Alexander, A.Sette, M.E.Grigg, A.Maewal, and R.McLeod (2010).
Identification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans.

Vaccine, 28, 3977-3989.

19553306

A.Wahl, W.McCoy, F.Schafer, W.Bardet, R.Buchli, D.H.Fremont, and W.H.Hildebrand (2009).
T-cell tolerance for variability in an HLA class I-presented influenza A virus epitope.

J Virol, 83, 9206-9214.

19617632

P.Kumar, A.Vahedi-Faridi, W.Saenger, E.Merino, J.A.López de Castro, B.Uchanska-Ziegler, and A.Ziegler (2009).
Structural basis for T cell alloreactivity among three HLA-B14 and HLA-B27 antigens.

J Biol Chem, 284, 29784-29797.

PDB codes:

3bp4 3bp7 3bvn 3bxn

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.