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PDBsum entry 2ceb
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Fertilization PDB id
2ceb

 

 

 

 

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Contents
Protein chain
89 a.a.
Theoretical model
PDB id:
2ceb
Name: Fertilization
Title: Bovine seminal plasma protein bsp-30
Structure: Seminal plasma protein bsp-30 kda. Synonym: bsp-30k. Chain: a. Fragment: fibronectin-type2, residues 95-183
Source: Bos taurus. Bovine. Tissue: male accessory glands
Authors: T.M.Gwathmey,G.G.Ignotz,J.L.Mueller,P.Manjunath,S.S.Suarez
Key ref: T.M.Gwathmey et al. (2006). Bovine seminal plasma proteins PDC-109, BSP-A3, and BSP-30-kDa share functional roles in storing sperm in the oviduct. Biol Reprod, 75, 501-507. PubMed id: 16790686
Date:
03-Feb-06     Release date:   07-Feb-06    
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P81019  (SFP4_BOVIN) -  Seminal plasma protein BSP-30 kDa
Seq:
Struc: 		183 a.a.
89 a.a.
Key:    PfamA domain  Secondary structure

 

 
Biol Reprod 75:501-507 (2006)
PubMed id: 16790686  
 
 
Bovine seminal plasma proteins PDC-109, BSP-A3, and BSP-30-kDa share functional roles in storing sperm in the oviduct.
T.M.Gwathmey, G.G.Ignotz, J.L.Mueller, P.Manjunath, S.S.Suarez.
 
  ABSTRACT  
 
On ejaculation, sperm become coated with proteins secreted by the male accessory sex glands. In the bull, these proteins consist predominantly of the bovine seminal plasma family of proteins (BSPs): PDC-109 (BSP-A1/-A2), BSP-A3, and BSP-30-kDa. PDC-109 plays a role in forming an oviductal sperm reservoir by enabling sperm to bind to oviductal epithelium. Because PDC-109 has high sequence identity with the other BSPs, we tested BSP-A3 and BSP-30-kDa for the capacity to bind sperm to oviductal epithelium. BSP-A3 and BSP-30-kDa each increased binding of epididymal sperm to epithelium and were as effective as PDC-109 in competitively inhibiting binding of ejaculated sperm. Because binding extends the motile life of sperm, BSPs were tested for the ability to maintain sperm motility. BSP-treated epididymal sperm incubated with plasma membrane vesicles from bovine oviductal epithelium maintained progressive motility longer than untreated sperm. To our knowledge, this is the first report of this protective effect of BSPs. Similarities in function among the BSPs were reflected in their three-dimensional structure, whereas surface maps of electrostatic potential indicated differences in binding affinities and kinetics. Such differences may provide sperm with greater adaptability to variations among females. Altogether, these results indicate that BSPs play a crucial role in fertilization by maintaining sperm motility during storage.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20174627 H.J.Kim, M.Y.Choi, H.J.Kim, and M.Llinás (2010).
Conformational dynamics and ligand binding in the multi-domain protein PDC109.
  PLoS One, 5, e9180.  
20849222 J.L.Bailey (2010).
Factors regulating sperm capacitation.
  Syst Biol Reprod Med, 56, 334-348.  
20591333 M.E.Beltman, N.Forde, P.Furney, F.Carter, J.F.Roche, P.Lonergan, and M.A.Crowe (2010).
Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination.
  Reprod Fertil Dev, 22, 987-999.  
19210926 K.Ho, C.A.Wolff, and S.S.Suarez (2009).
CatSper-null mutant spermatozoa are unable to ascend beyond the oviductal reservoir.
  Reprod Fertil Dev, 21, 345-350.  
19349638 M.F.Wolfner (2009).
Battle and ballet: molecular interactions between the sexes in Drosophila.
  J Hered, 100, 399-410.  
18757944 A.Wong, S.N.Albright, J.D.Giebel, K.R.Ram, S.Ji, A.C.Fiumera, and M.F.Wolfner (2008).
A role for Acp29AB, a predicted seminal fluid lectin, in female sperm storage in Drosophila melanogaster.
  Genetics, 180, 921-931.  
18638148 J.P.Kastelic, and J.C.Thundathil (2008).
Breeding soundness evaluation and semen analysis for predicting bull fertility.
  Reprod Domest Anim, 43, 368-373.  
18653675 S.S.Suarez (2008).
Control of hyperactivation in sperm.
  Hum Reprod Update, 14, 647-657.  
18658160 T.L.Tollner, A.I.Yudin, C.A.Treece, J.W.Overstreet, and G.N.Cherr (2008).
Macaque sperm coating protein DEFB126 facilitates sperm penetration of cervical mucus.
  Hum Reprod, 23, 2523-2534.  
17612869 M.L.Vadnais, H.L.Galantino-Homer, and G.C.Althouse (2007).
Current concepts of molecular events during bovine and porcine spermatozoa capacitation.
  Arch Androl, 53, 109-123.  
17389139 S.S.Suarez (2007).
Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction.
  Reprod Fertil Dev, 19, 103-110.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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