 |
PDBsum entry 2ccx
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Eur J Biochem
213:891-900
(1993)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Determination of the nuclear-magnetic-resonance solution structure of cardiotoxin CTX IIb from Naja mossambica mossambica.
|
|
J.F.O'Connell,
P.E.Bougis,
K.Wüthrich.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
The NMR structure of cardiotoxin CTX IIb from Naja mossambica mossambica in
aqueous solution was determined from a total of 593 nuclear Overhauser
enhancement distance constraints and 135 dihedral angle constraints, which were
collected using two-dimensional homonuclear 1H-NMR experiments. Structure
calculations were performed with the program DIANA, using the redundant dihedral
angle constraints strategy for improved convergence, followed by restrained
energy minimization with the program FANTOM and a modified version of the
program AMBER. The CTX IIb structure is represented by a group of 20 conformers
with an average root-mean-square deviation relative to the mean solution
structure of 0.072 nm for the backbone atoms, and 0.116 nm for all heavy atoms.
The molecular structure of CTX IIb is characterized by a three-stranded
beta-sheet made up of residues 20-26, 32-39 and 48-54, and a two-stranded
beta-sheet composed of residues 1-5 and 10-14. A cluster of four disulfide
bonds, 3-21, 14-38, 42-53 and 54-59, form the core of the molecule and crosslink
the individual polypeptide strands. The NMR structure is similar to the
previously reported X-ray crystal structure of the cardiotoxin CTX VII4 from the
same species. Differences between the two structures were noted in the tips of
the two loops formed by residues 6-9 and 27-31, which connect the beta-strand
1-5 with 10-14, and 20-26 with 32-39, respectively. For these loops the NMR data
also indicate significantly increased dynamic disorder in the solution
structure. These observations are discussed with respect to earlier suggestions
by others that these two loops are essential structural elements for function
and specificity of a wide variety of homologous toxins.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
I.Hudáky,
and
A.Perczel
(2008).
Prolylproline unit in model peptides and in fragments from databases.
|
| |
Proteins,
70,
1389-1407.
|
|
|
|
|
|
|
D.V.Dementieva,
E.V.Bocharov,
and
A.S.Arseniev
(1999).
Two forms of cytotoxin II (cardiotoxin) from Naja naja oxiana in aqueous solution: spatial structures with tightly bound water molecules.
|
| |
Eur J Biochem,
263,
152-162.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
C.C.Lo,
J.H.Hsu,
Y.C.Sheu,
C.M.Chiang,
W.Wu,
W.Fann,
and
P.H.Tsao
(1998).
Effect of D57N mutation on membrane activity and molecular unfolding of cobra cardiotoxin.
|
| |
Biophys J,
75,
2382-2388.
|
|
|
|
|
|
|
P.A.Carr,
H.P.Erickson,
and
A.G.Palmer
(1997).
Backbone dynamics of homologous fibronectin type III cell adhesion domains from fibronectin and tenascin.
|
| |
Structure,
5,
949-959.
|
|
|
|
|
|
|
F.Picard,
M.Pézolet,
P.E.Bougis,
and
M.Auger
(1996).
Model of interaction between a cardiotoxin and dimyristoylphosphatidic acid bilayers determined by solid-state 31P NMR spectroscopy.
|
| |
Biophys J,
70,
1737-1744.
|
|
|
|
|
|
|
M.Dauplais,
J.M.Neumann,
S.Pinkasfeld,
A.Ménez,
and
C.Roumestand
(1995).
An NMR study of the interaction of cardiotoxin gamma from Naja nigricollis with perdeuterated dodecylphosphocholine micelles.
|
| |
Eur J Biochem,
230,
213-220.
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
|
|
Links
