PDBsum entry 2c4l

Hydrolase

PDB id: 2c4l

Contents

Protein chain

388 a.a. *

Ligands

MAN-MAN

SIA ×2

NAG-NAG-BMA

BMA ×4

NAG-NAG

NAG

Metals

_CA

Waters ×233

* Residue conservation analysis

PDB id:

2c4l

Name:

Hydrolase

Title:

Structure of neuraminidase subtype n9 complexed with 30 mm sialic acid (nana, neu5ac), crystal soaked for 24 hours at 291 k and finally backsoaked for 30 min in a cryoprotectant solution which did not contain neu5ac

Structure:

Neuraminidase subtype n9. Chain: a. Fragment: residues 83-470. Engineered: yes

Source:

Influenza a virus. Avian influenza virus a. Organism_taxid: 11320. Strain: a/tern/sterna albifrons/n9. Expressed in: gallus gallus. Expression_system_taxid: 9031

Resolution:

2.15Å R-factor: 0.157 R-free: 0.196

Authors:

E.Rudino-Pinera,P.Tunnah,S.J.Crennell,R.G.Webster,W.G.Laver, E.F.Garman

Key ref:

E.Rudino-Pinera et al. The crystal structure of influenza type a virus neuraminidase of the n6 subtype at 1.85 a resolution. To be published, .

Date:

20-Oct-05 Release date: 27-Mar-07

Protein chain

P03472  (NRAM_I75A5) -  Neuraminidase from Influenza A virus (strain A/Tern/Australia/G70C/1975 H11N9)

Seq: 470 a.a.

Struc: 388 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.1.18 - exo-alpha-sialidase.
• Reaction: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)-glycosidic linkages of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.