PDBsum entry 2b6a

Transferase

PDB id: 2b6a

Contents

Protein chains

558 a.a. *

427 a.a. *

Ligands

T50

* Residue conservation analysis

PDB id:

2b6a

Name:

Transferase

Title:

Crystal structure of HIV-1 reverse transcriptase (rt) in complex with thr-50

Structure:

Reverse transcriptase p66 subunit. Chain: a. Fragment: residues 599-1158. Synonym: HIV-1 rt. Engineered: yes. Mutation: yes. Reverse transcriptase p51 subunit. Chain: b. Fragment: residues 599-1028.

Source:

Human immunodeficiency virus 1. Organism_taxid: 11676. Gene: pol. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

2.65Å R-factor: 0.226 R-free: 0.277

Authors:

M.L.Morningstar,T.Roth,M.K.Smith,M.Zajac,K.Watson,R.W.Buckheit,K.Das, W.Zhang,E.Arnold,C.J.Michejda

Key ref:

M.L.Morningstar et al. Crystal structure of HIV-1 reverse transcriptase (rt) in complex with thr-50. To be published, .

Date:

30-Sep-05 Release date: 01-Nov-05

Protein chain

P03366  (POL_HV1B1) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10)

Seq: 1447 a.a.

Struc: 558 a.a.*

Protein chain

P03366  (POL_HV1B1) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10)

Seq: 1447 a.a.

Struc: 427 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.2.7.7.- - ?????
• Enzyme class 2: Chains A, B: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: Chains A, B: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: Chains A, B: E.C.3.1.-.-
• Enzyme class 5: Chains A, B: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: Chains A, B: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 7: Chains A, B: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site