PDBsum entry 2asv

Transferase

PDB id: 2asv

Contents

Protein chains

796 a.a. *

Ligands

BGC-GLC-GLC-GLC-GLC ×2

ASO ×2

PO4 ×2

PLP ×2

Waters ×1184

* Residue conservation analysis

PDB id:

2asv

Name:

Transferase

Title:

X-ray studies on protein complexes: enzymatic catalysis in crystals of e. Coli maltodextrin phosphorylase (malp)

Structure:

Maltodextrin phosphorylase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: eg10560. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

1.95Å R-factor: 0.182 R-free: 0.226

Authors:

S.Geremia,M.Campagnolo

Key ref:

S.Geremia and m.campagnolo X-Ray studies on protein complexes: enzymatic catalysis in crystals of e.Coli maltodextrin phosphorylase (malp). To be published, .

Date:

24-Aug-05 Release date: 06-Sep-05

Protein chains

P00490  (PHSM_ECOLI) -  Maltodextrin phosphorylase from Escherichia coli (strain K12)

Seq: 797 a.a.

Struc: 796 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.4.1.1 - glycogen phosphorylase.
• Pathway: Glycogen
• Reaction: [(1->4)-alpha-D-glucosyl](n) + phosphate = [(1->4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate

[(1->4)-alpha-D-glucosyl](n) + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly) = [(1->4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate (Bound ligand (Het Group name = BGC) matches with 75.00% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site