PDBsum entry 2zwf

Oxidoreductase/metal transport

PDB id: 2zwf

Contents

Protein chains

276 a.a. *

72 a.a. *

Ligands

NO3 ×5

Metals

_CU ×4

Waters ×424

* Residue conservation analysis

PDB id:

2zwf

Name:

Oxidoreductase/metal transport

Title:

Crystal structure of the copper-bound tyrosinase in complex with a caddie protein from streptomyces castaneoglobisporus obtained by soaking the deoxy-form crystal in dioxygen-saturated solution for 80 minutes

Structure:

Tyrosinase. Chain: a. Engineered: yes. Melc. Chain: b. Synonym: caddie protein orf378. Engineered: yes

Source:

Streptomyces castaneoglobisporus. Organism_taxid: 79261. Strain: hut 6202. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.40Å R-factor: 0.173 R-free: 0.212

Authors:

Y.Matoba,M.Sugiyama

Key ref:

Y.Matoba et al. Crystallographic evidence of drastic movement of a copper ion toward the substrate tyrosine for starting hydroxylation reaction of tyrosinase. To be published, .

Date:

03-Dec-08 Release date: 15-Dec-09

Protein chain

Q83WS2  (Q83WS2_9ACTN) -  Tyrosinase from Streptomyces castaneoglobisporus

Seq: 273 a.a.

Struc: 276 a.a.*

Protein chain

Q83WS1  (Q83WS1_9ACTN) -  MelC from Streptomyces castaneoglobisporus

Seq: 126 a.a.

Struc: 72 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.1.14.18.1 - tyrosinase.
• Pathway: Melanin Biosynthesis
• Reaction:
  1. L-tyrosine + O2 = L-dopaquinone + H2O
  2. 2 L-dopa + O2 = 2 L-dopaquinone + 2 H2O
• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site