PDBsum entry 2z02

Ligase

PDB id: 2z02

Contents

Protein chains

242 a.a. *

Ligands

SO4 ×3

ATP ×2

CIT

Waters ×299

* Residue conservation analysis

PDB id:

2z02

Name:

Ligase

Title:

Crystal structure of phosphoribosylaminoimidazolesuccinocarboxamide synthase wit atp from methanocaldococcus jannaschii

Structure:

Phosphoribosylaminoimidazole-succinocarboxamide synthase. Chain: a, b. Synonym: saicar synthetase. Engineered: yes

Source:

Methanocaldococcus jannaschii. Organism_taxid: 2190. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.03Å R-factor: 0.207 R-free: 0.217

Authors:

M.Kanagawa,S.Baba,S.Kuramitsu,S.Yokoyama,G.Kawai,G.Sampei,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

M.Kanagawa et al. Crystal structure of phosphoribosylaminoimidazolesuccinocarboxamide synthase from methanocaldococcus jannaschii. To be published, .

Date:

06-May-07 Release date: 06-Nov-07

Protein chains

Q58987  (PUR7_METJA) -  Phosphoribosylaminoimidazole-succinocarboxamide synthase from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Seq: 242 a.a.

Struc: 242 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.2.6 - phosphoribosylaminoimidazolesuccinocarboxamide synthase.
• Pathway: Purine Biosynthesis (late stages)
• Reaction: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4- carboxamido]succinate + ADP + phosphate + 2 H⁺

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate (Bound ligand (Het Group name = ATP) corresponds exactly) + ATP (Bound ligand (Het Group name = CIT) matches with 57.14% similarity) = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4- carboxamido]succinate + ADP + phosphate + 2 × H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site