PDBsum entry 2y8s

Membrane protein

PDB id: 2y8s

Contents

Protein chains

386 a.a.

36 a.a.

35 a.a.

Ligands

NAG ×2

BO3 ×2

GOL ×3

Waters ×211

PDB id:

2y8s

Name:

Membrane protein

Title:

Co-structure of an ama1 mutant (y230a) with a surface exposed region of ron2 from toxoplasma gondii

Structure:

Apical membrane antigen, putative. Chain: a, d. Fragment: domains i/ii/iii, residues 64-484. Synonym: ama1, apical membrane antigen 1. Engineered: yes. Mutation: yes. Rhoptry neck protein 2. Chain: b, e. Fragment: residues 235-271.

Source:

Toxoplasma gondii. Organism_taxid: 5811. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: high five. Synthetic: yes. Organism_taxid: 5811

Resolution:

2.55Å R-factor: 0.189 R-free: 0.263

Authors:

M.L.Tonkin,M.Roques,M.H.Lamarque,M.Pugniere,D.Douguet,J.Crawford, M.Lebrun,M.J.Boulanger

Key ref:

M.L.Tonkin et al. (2011). Host cell invasion by apicomplexan parasites: insights from the co-structure of AMA1 with a RON2 peptide. Science, 333, 463-467. PubMed id: 21778402

Date:

10-Feb-11 Release date: 03-Aug-11

Protein chains

B6KAM0  (AMA1_TOXGV) -  Apical membrane antigen 1 from Toxoplasma gondii (strain ATCC 50861 / VEG)

Seq: 569 a.a.

Struc: 386 a.a.*

Protein chain

B6KV60  (RON2_TOXGM) -  Rhoptry neck protein 2 from Toxoplasma gondii (strain ATCC 50611 / Me49)

Seq: 1479 a.a.

Struc: 36 a.a.

Protein chain

B6KV60  (RON2_TOXGM) -  Rhoptry neck protein 2 from Toxoplasma gondii (strain ATCC 50611 / Me49)

Seq: 1479 a.a.

Struc: 35 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Science 333:463-467 (2011)

PubMed id: 21778402

Host cell invasion by apicomplexan parasites: insights from the co-structure of AMA1 with a RON2 peptide.

M.L.Tonkin, M.Roques, M.H.Lamarque, M.Pugnière, D.Douguet, J.Crawford, M.Lebrun, M.J.Boulanger.

ABSTRACT

No abstract given.