PDBsum entry 2y7e

Lyase

PDB id: 2y7e

Contents

Protein chains

274 a.a.

Ligands

FMT ×2

Metals

_MG

_ZN ×2

Waters ×585

PDB id:

2y7e

Name:

Lyase

Title:

Crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme (kce) from candidatus cloacamonas acidaminovorans (tetragonal form)

Structure:

3-keto-5-aminohexanoate cleavage enzyme. Chain: a, b. Fragment: residues 2-276. Engineered: yes

Source:

Candidatus cloacamonas acidaminovorans. Organism_taxid: 456827. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: plyss.

Resolution:

1.28Å R-factor: 0.145 R-free: 0.170

Authors:

M.Bellinzoni,P.M.Alzari

Key ref:

M.Bellinzoni et al. (2011). 3-Keto-5-aminohexanoate cleavage enzyme: a common fold for an uncommon Claisen-type condensation. J Biol Chem, 286, 27399-27405. PubMed id: 21632536

Date:

31-Jan-11 Release date: 01-Jun-11

Protein chains

B0VHH0  (KCE_CLOAI) -  3-keto-5-aminohexanoate cleavage enzyme from Cloacimonas acidaminovorans (strain Evry)

Seq: 276 a.a.

Struc: 274 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.1.247 - (5S)-5-amino-3-oxohexanoate:acetyl-CoA ethylamine transferase.
• Reaction: (5S)-5-amino-3-oxohexanoate + acetyl-CoA = (3S)-3-aminobutanoyl-CoA + acetoacetate

(5S)-5-amino-3-oxohexanoate + acetyl-CoA = (3S)-3-aminobutanoyl-CoA + acetoacetate (Bound ligand (Het Group name = FMT) matches with 42.86% similarity)

• Cofactor: Zn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Biol Chem 286:27399-27405 (2011)

PubMed id: 21632536

3-Keto-5-aminohexanoate cleavage enzyme: a common fold for an uncommon Claisen-type condensation.

M.Bellinzoni, K.Bastard, A.Perret, A.Zaparucha, N.Perchat, C.Vergne, T.Wagner, R.C.de Melo-Minardi, F.Artiguenave, G.N.Cohen, J.Weissenbach, M.Salanoubat, P.M.Alzari.

ABSTRACT

No abstract given.