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PDBsum entry 2xm1
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PDB id:
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Hydrolase
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Title:
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Btgh84 in complex with n-acetyl gluconolactam
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Structure:
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O-glcnacase bt_4395. Chain: a, b. Synonym: btgh84, beta-hexosaminidase, hexosaminidase b, gh84, n- acetyl-beta-glucosaminidase, beta-n-acetylhexosaminidase,. Engineered: yes
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Source:
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Bacteroides thetaiotaomicron vpi-5482. Organism_taxid: 226186. Atcc: 29148. Expressed in: escherichia coli. Expression_system_taxid: 511693.
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Resolution:
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2.00Å
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R-factor:
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0.182
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R-free:
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0.220
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Authors:
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Y.He,G.J.Davies
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Key ref:
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Y.He
et al.
(2011).
Inhibition of a bacterial O-GlcNAcase homologue by lactone and lactam derivatives: structural, kinetic and thermodynamic analyses.
Amino Acids,
40,
829-839.
PubMed id:
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Date:
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22-Jul-10
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Release date:
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03-Aug-11
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PROCHECK
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Headers
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References
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Q89ZI2
(OGA_BACTN) -
O-GlcNAcase BT_4395 from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
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Seq:
Struc:
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737 a.a.
645 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.3.2.1.169
- protein O-GlcNAcase.
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Reaction:
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1.
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3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H2O = N-acetyl-D-glucosamine + L-seryl-[protein]
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2.
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3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H2O = L-threonyl-[protein] + N-acetyl-D-glucosamine
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3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein]
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+
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H2O
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=
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N-acetyl-D-glucosamine
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+
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L-seryl-[protein]
Bound ligand (Het Group name = )
matches with 87.50% similarity
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3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein]
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+
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H2O
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=
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L-threonyl-[protein]
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+
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N-acetyl-D-glucosamine
Bound ligand (Het Group name = )
matches with 87.50% similarity
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Amino Acids
40:829-839
(2011)
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PubMed id:
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Inhibition of a bacterial O-GlcNAcase homologue by lactone and lactam derivatives: structural, kinetic and thermodynamic analyses.
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Y.He,
A.K.Bubb,
K.A.Stubbs,
T.M.Gloster,
G.J.Davies.
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ABSTRACT
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