PDBsum entry 2xci

Transferase

PDB id: 2xci

Contents

Protein chains

354 a.a.

Ligands

PG4 ×4

GOL ×8

BME ×2

Metals

_CL ×8

_NI ×4

Waters ×248

PDB id:

2xci

Name:

Transferase

Title:

Membrane-embedded monofunctional glycosyltransferase waaa of aquifex aeolicus, substrate-free form

Structure:

3-deoxy-d-manno-2-octulosonic acid transferase. Chain: a, b, c, d. Synonym: kdo-transferase. Engineered: yes

Source:

Aquifex aeolicus. Organism_taxid: 63363. Strain: aq_326. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: si-216.

Resolution:

2.00Å R-factor: 0.210 R-free: 0.254

Authors:

H.Schmidt,G.Hansen,R.Hilgenfeld,U.Mamat,J.R.Mesters

Key ref:

H.Schmidt et al. (2012). Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis. Proc Natl Acad Sci U S A, 109, 6253-6258. PubMed id: 22474366

Date:

26-Apr-10 Release date: 11-May-11

Protein chains

O66663  (KDTA_AQUAE) -  3-deoxy-D-manno-octulosonic acid transferase from Aquifex aeolicus (strain VF5)

Seq: 353 a.a.

Struc: 354 a.a.

Enzyme reactions

• Enzyme class: E.C.2.4.99.12 - lipid Iva 3-deoxy-D-manno-octulosonic acid transferase.
• Reaction: a lipid IVA + CMP-3-deoxy-beta-D-manno-octulosonate = an alpha- Kdo-(2->6)-lipid IVA + CMP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

Proc Natl Acad Sci U S A 109:6253-6258 (2012)

PubMed id: 22474366

Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis.

H.Schmidt, G.Hansen, S.Singh, A.Hanuszkiewicz, B.Lindner, K.Fukase, R.W.Woodard, O.Holst, R.Hilgenfeld, U.Mamat, J.R.Mesters.

ABSTRACT

No abstract given.