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PDBsum entry 2ws3
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protein ligands metals Protein-protein interface(s) links
Oxidoreductase PDB id
2ws3

 

 

 

 

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Contents
Protein chains
588 a.a. *
238 a.a. *
121 a.a. *
105 a.a. *
Ligands
FAD ×3
TEO ×3
FES ×3
SF4 ×3
F3S ×3
CBE ×3
HEM ×3
Metals
_NA ×3
* Residue conservation analysis
PDB id:
2ws3
Name: Oxidoreductase
Title: Crystal structure of the e. Coli succinate:quinone oxidoreductase (sqr) sdhd tyr83phe mutant
Structure: Succinate dehydrogenase flavoprotein subunit. Chain: a, e, i. Engineered: yes. Other_details: fad atom c8m is covalently linked to ne2 of sdha his45. Succinate dehydrogenase iron-sulfur subunit. Chain: b, f, j. Engineered: yes. Succinate dehydrogenase cytochrome b-556 subunit.
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
3.20Å     R-factor:   0.220     R-free:   0.253
Authors: J.Ruprecht,V.Yankovskaya,E.Maklashina,S.Iwata,G.Cecchini
Key ref: J.Ruprecht et al. Succinate dehydrogenase activity. To be published, .
Date:
03-Sep-09     Release date:   25-Aug-10    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P0AC41  (SDHA_ECOLI) -  Succinate dehydrogenase flavoprotein subunit from Escherichia coli (strain K12)
Seq:
Struc: 		 
Seq:
Struc: 		588 a.a.
588 a.a.
Protein chains
Pfam   ArchSchema ?
P07014  (SDHB_ECOLI) -  Succinate dehydrogenase iron-sulfur subunit from Escherichia coli (strain K12)
Seq:
Struc: 		238 a.a.
238 a.a.
Protein chains
Pfam   ArchSchema ?
P69054  (DHSC_ECOLI) -  Succinate dehydrogenase cytochrome b556 subunit from Escherichia coli (strain K12)
Seq:
Struc: 		129 a.a.
121 a.a.
Protein chains
Pfam   ArchSchema ?
P0AC44  (DHSD_ECOLI) -  Succinate dehydrogenase hydrophobic membrane anchor subunit from Escherichia coli (strain K12)
Seq:
Struc: 		115 a.a.
105 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D, E, F, G, H, I, J, K, L: E.C.1.3.5.1  - succinate dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway: 		Citric acid cycle
      Reaction: a quinone + succinate = fumarate + a quinol
quinone
 +
succinate
Bound ligand (Het Group name = TEO)
matches with 88.89% similarity 		= fumarate
 + quinol
      Cofactor: FAD; Iron-sulfur
FAD
Bound ligand (Het Group name = FAD) corresponds exactly 		Iron-sulfur
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

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