PDBsum entry 2vz6

Transferase

PDB id: 2vz6

Contents

Protein chains

291 a.a. *

Ligands

FEF ×2

PGO ×3

Waters ×311

* Residue conservation analysis

PDB id:

2vz6

Name:

Transferase

Title:

Structure of human calcium calmodulin dependent protein kinase type ii alpha (camk2a) in complex with indirubin e804

Structure:

Calcium calmodulin dependent protein kinase type ii alpha chain. Chain: a, b. Fragment: kinase domain, residues 13-302. Synonym: cam-kinase ii alpha chain, cam kinase ii subunit alpha, camk-ii subunit alpha. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: r3-prare2.

Resolution:

2.30Å R-factor: 0.165 R-free: 0.203

Authors:

A.C.W.Pike,P.Rellos,O.King,E.Salah,E.Parizotto,O.Fedorov,L.Shrestha, N.Burgess-Brown,A.Roos,J.W.Murray,F.Von Delft,A.Edwards, C.H.Arrowsmith,M.Wikstroem,C.Bountra,S.Knapp

Key ref:

P.Rellos et al. (2010). Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation. Plos Biol, 8, e1000426. PubMed id: 20668654

Date:

30-Jul-08 Release date: 26-Aug-08

Protein chains

Q9UQM7  (KCC2A_HUMAN) -  Calcium/calmodulin-dependent protein kinase type II subunit alpha from Homo sapiens

Seq: 478 a.a.

Struc: 291 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.17 - calcium/calmodulin-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
• Cofactor: Ca(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos Biol 8:e1000426 (2010)

PubMed id: 20668654

Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation.

P.Rellos, A.C.Pike, F.H.Niesen, E.Salah, W.H.Lee, F.von Delft, S.Knapp.

ABSTRACT

No abstract given.