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PDBsum entry 2vvy
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Viral protein
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PDB id
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2vvy
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Contents |
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* Residue conservation analysis
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PDB id:
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Viral protein
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Title:
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Structure of vaccinia virus protein b14
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Structure:
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Protein b15. Chain: a, b, c, d. Synonym: b14. Engineered: yes
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Source:
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Vaccinia virus. Organism_taxid: 10245. Strain: western reserve. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.69Å
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R-factor:
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0.224
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R-free:
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0.244
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Authors:
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S.C.Graham,M.W.Bahar,S.Cooray,R.A.-J.Chen,D.M.Whalen,N.G.A.Abrescia, D.Alderton,R.J.Owens,D.I.Stuart,G.L.Smith,J.M.Grimes
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Key ref:
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S.C.Graham
et al.
(2008).
Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis.
Plos Pathog,
4,
e1000128.
PubMed id:
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Date:
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12-Jun-08
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Release date:
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26-Aug-08
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PROCHECK
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Headers
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References
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P24772
(B14_VACCW) -
Protein OPG200 from Vaccinia virus (strain Western Reserve)
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Seq:
Struc:
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149 a.a.
142 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Plos Pathog
4:e1000128
(2008)
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PubMed id:
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Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis.
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S.C.Graham,
M.W.Bahar,
S.Cooray,
R.A.Chen,
D.M.Whalen,
N.G.Abrescia,
D.Alderton,
R.J.Owens,
D.I.Stuart,
G.L.Smith,
J.M.Grimes.
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ABSTRACT
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Vaccinia virus (VACV), the prototype poxvirus, encodes numerous proteins that
modulate the host response to infection. Two such proteins, B14 and A52, act
inside infected cells to inhibit activation of NF-kappaB, thereby blocking the
production of pro-inflammatory cytokines. We have solved the crystal structures
of A52 and B14 at 1.9 A and 2.7 A resolution, respectively. Strikingly, both
these proteins adopt a Bcl-2-like fold despite sharing no significant sequence
similarity with other viral or cellular Bcl-2-like proteins. Unlike cellular and
viral Bcl-2-like proteins described previously, A52 and B14 lack a surface
groove for binding BH3 peptides from pro-apoptotic Bcl-2-like proteins and they
do not modulate apoptosis. Structure-based phylogenetic analysis of 32 cellular
and viral Bcl-2-like protein structures reveals that A52 and B14 are more
closely related to each other and to VACV N1 and myxoma virus M11 than they are
to other viral or cellular Bcl-2-like proteins. This suggests that a progenitor
poxvirus acquired a gene encoding a Bcl-2-like protein and, over the course of
evolution, gene duplication events have allowed the virus to exploit this Bcl-2
scaffold for interfering with distinct host signalling pathways.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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D.Coulson,
and
C.Upton
(2011).
Characterization of indels in poxvirus genomes.
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Virus Genes,
42,
171-177.
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M.M.Rahman,
and
G.McFadden
(2011).
Modulation of NF-κB signalling by microbial pathogens.
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Nat Rev Microbiol,
9,
291-306.
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S.J.Hansen,
J.Rushton,
A.Dekonenko,
H.S.Chand,
G.K.Olson,
J.A.Hutt,
D.Pickup,
C.R.Lyons,
and
M.F.Lipscomb
(2011).
Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection.
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Virology,
412,
411-425.
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D.G.Diel,
G.Delhon,
S.Luo,
E.F.Flores,
and
D.L.Rock
(2010).
A novel inhibitor of the NF-{kappa}B signaling pathway encoded by the parapoxvirus orf virus.
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J Virol,
84,
3962-3973.
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G.W.Morgan,
M.Hollinshead,
B.J.Ferguson,
B.J.Murphy,
D.C.Carpentier,
and
G.L.Smith
(2010).
Vaccinia protein F12 has structural similarity to kinesin light chain and contains a motor binding motif required for virion export.
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PLoS Pathog,
6,
e1000785.
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J.M.González,
and
M.Esteban
(2010).
A poxvirus Bcl-2-like gene family involved in regulation of host immune response: sequence similarity and evolutionary history.
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Virol J,
7,
59.
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L.E.McCoy,
A.S.Fahy,
R.A.Chen,
and
G.L.Smith
(2010).
Mutations in modified virus Ankara protein 183 render it a non-functional counterpart of B14, an inhibitor of nuclear factor kappaB activation.
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J Gen Virol,
91,
2216-2220.
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B.Perdiguero,
and
M.Esteban
(2009).
The interferon system and vaccinia virus evasion mechanisms.
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J Interferon Cytokine Res,
29,
581-598.
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C.Myskiw,
J.Arsenio,
R.van Bruggen,
Y.Deschambault,
and
J.Cao
(2009).
Vaccinia virus E3 suppresses expression of diverse cytokines through inhibition of the PKR, NF-kappaB, and IRF3 pathways.
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J Virol,
83,
6757-6768.
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K.Van Vliet,
M.R.Mohamed,
L.Zhang,
N.Y.Villa,
S.J.Werden,
J.Liu,
and
G.McFadden
(2009).
Poxvirus proteomics and virus-host protein interactions.
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Microbiol Mol Biol Rev,
73,
730-749.
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L.Banadyga,
K.Veugelers,
S.Campbell,
and
M.Barry
(2009).
The fowlpox virus BCL-2 homologue, FPV039, interacts with activated Bax and a discrete subset of BH3-only proteins to inhibit apoptosis.
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J Virol,
83,
7085-7098.
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M.M.Rahman,
M.R.Mohamed,
M.Kim,
S.Smallwood,
and
G.McFadden
(2009).
Co-regulation of NF-kappaB and inflammasome-mediated inflammatory responses by myxoma virus pyrin domain-containing protein m013.
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PLoS Pathog,
5,
e1000635.
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N.C.Elde,
and
H.S.Malik
(2009).
The evolutionary conundrum of pathogen mimicry.
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Nat Rev Microbiol,
7,
787-797.
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S.J.Chang,
J.C.Hsiao,
S.Sonnberg,
C.T.Chiang,
M.H.Yang,
D.L.Tzou,
A.A.Mercer,
and
W.Chang
(2009).
Poxvirus host range protein CP77 contains an F-box-like domain that is necessary to suppress NF-kappaB activation by tumor necrosis factor alpha but is independent of its host range function.
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J Virol,
83,
4140-4152.
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S.Oda,
M.Schröder,
and
A.R.Khan
(2009).
Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7.
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Structure,
17,
1528-1537.
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PDB code:
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T.A.Bowden,
A.R.Aricescu,
J.E.Nettleship,
C.Siebold,
N.Rahman-Huq,
R.J.Owens,
D.I.Stuart,
and
E.Y.Jones
(2009).
Structural plasticity of eph receptor A4 facilitates cross-class ephrin signaling.
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Structure,
17,
1386-1397.
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PDB codes:
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T.H.Mogensen
(2009).
Pathogen recognition and inflammatory signaling in innate immune defenses.
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Clin Microbiol Rev,
22,
240.
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A.Mathew,
J.O'Bryan,
W.Marshall,
G.J.Kotwal,
M.Terajima,
S.Green,
A.L.Rothman,
and
F.A.Ennis
(2008).
Robust intrapulmonary CD8 T cell responses and protection with an attenuated N1L deleted vaccinia virus.
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PLoS ONE,
3,
e3323.
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A.S.Fahy,
R.H.Clark,
E.F.Glyde,
and
G.L.Smith
(2008).
Vaccinia virus protein C16 acts intracellularly to modulate the host response and promote virulence.
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J Gen Virol,
89,
2377-2387.
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N.Jacobs,
N.W.Bartlett,
R.H.Clark,
and
G.L.Smith
(2008).
Vaccinia virus lacking the Bcl-2-like protein N1 induces a stronger natural killer cell response to infection.
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J Gen Virol,
89,
2877-2881.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
